Hotspot

ycf4

LSC · 60081-60656 · 576 bp

Back to Adenocalymma

Pi 0.148

MarkerSeek score 83.10

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 576
variable_sites: 298
pi: 0.148
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	TTCAAATAGATCAGGGGCGC	TGGCTCGACTGAATGTTCTG	50.0	50.0	58.0	57.9	910-990 bp	0.833333	84.4
ycf4_p2	GGAAAAAGCGCTTATGCCTG	TGCATGGATTTGGATACGCA	50.0	45.0	58.1	58.0	995-1123 bp	0.833333	84.2
ycf4_p3	TTCAAATAGATCAGGGGCGC	TGCATGGATTTGGATACGCA	50.0	45.0	58.0	58.0	992-1066 bp	0.75	81.3
ycf4_p4	TTCAAATAGATCAGGGGCGC	ACAAGACAGAAATGGGCCAG	50.0	50.0	58.0	58.1	1242-1344 bp	0.75	81.1
ycf4_p5	TTCAAATAGATCAGGGGCGC	CAAGACAGAAATGGGCCAGT	50.0	50.0	58.0	58.1	1241-1343 bp	0.75	81.1