Hotspot

ycf1

IRb · 109607-115015 · 5409 bp

Back to Adenocalymma

Pi 0.0949

MarkerSeek score 77.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 5409
variable_sites: 2547
pi: 0.0949
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TACTTCTCCGAGCTGAGCTT	TCCGCCTTTTCTTCCGAAAA	50.0	45.0	58.2	58.0	817-823 bp	0.75	74.6
ycf1_p2	TTACTTCTCCGAGCTGAGCT	TCCGCCTTTTCTTCCGAAAA	50.0	45.0	58.2	58.0	818-824 bp	0.75	74.5
ycf1_p3	ACTTCTCCGAGCTGAGCTTA	TCCGCCTTTTCTTCCGAAAA	50.0	45.0	58.2	58.0	816 bp	0.583333	66.6
ycf1_p4	ACTTCTCCGAGCTGAGCTTA	CACTTTTGGGGGTTCTTCCA	50.0	50.0	58.2	57.9	679 bp	0.5	61.3
ycf1_p5	GGCAGGCCTCATACGATAAC	TCCGCCTTTTCTTCCGAAAA	55.0	45.0	58.2	58.0	692 bp	0.25	49.1