Hotspot

ccsA-ndhD

SSC · 120623-120854 · 232 bp

Back to Adenocalymma

Pi 0.0688

MarkerSeek score 79.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 232
variable_sites: 44
pi: 0.0688
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GAAGCATGGGGGTCCTATTG	TTTTGATTCGGGACCACGAG	55.0	50.0	58.0	58.2	602-616 bp	1.0	90.3
ccsA-ndhD_p2	GAAGCATGGGGGTCCTATTG	ATGGCTTCCCTTGCATTACC	55.0	50.0	58.0	58.2	817-831 bp	1.0	88.5
ccsA-ndhD_p3	GAAGCATGGGGGTCCTATTG	CTCAACGGAGCCCTTTTACA	55.0	50.0	58.0	57.8	982-996 bp	1.0	87.7
ccsA-ndhD_p4	GAAGCATGGGGGTCCTATTG	TGATGGTAGGTGCAATGCAA	55.0	45.0	58.0	57.8	1122-1136 bp	1.0	86.7
ccsA-ndhD_p5	CGGCCCTTTTATGTGGATCA	CTCAACGGAGCCCTTTTACA	50.0	50.0	57.9	57.8	1281-1295 bp	1.0	86.3