Hotspot

rpl32-trnL-UAC

SSC · 118608-119485 · 878 bp

Back to Adenocalymma

Pi 0.0325

MarkerSeek score 80.90

Primer available yes

Label rpl32-trnL-UAC

Feature Metadata

label_name: rpl32-trnL-UAC
feature_type: igs
region: SSC
length_bp: 878
variable_sites: 113
pi: 0.0325
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAC_p1	TTTTTGTGCGACAAACAAAT	CTCGCACTGCTTCCTAAGAG	30.0	55.0	53.4	58.1	957-1021 bp	1.0	54.2
rpl32-trnL-UAC_p2	TTTTTGTGCGACAAACAAAT	CTCTCGCACTGCTTCCTAAG	30.0	55.0	53.4	58.1	959-1023 bp	1.0	54.2
rpl32-trnL-UAC_p3	TTTTTGTGCGACAAACAAAT	TCCTAAGAGCAGCGTGTCTA	30.0	50.0	53.4	57.9	946-1010 bp	1.0	53.9
rpl32-trnL-UAC_p4	TTTTTGTGCGACAAACAAAT	AGCAGCGTGTCTACCAATTT	30.0	45.0	53.4	57.8	939-1003 bp	1.0	53.4
rpl32-trnL-UAC_p5	TTTTTGTGCGACAAACAAAT	CTAAGAGCAGCGTGTCTACC	30.0	55.0	53.4	57.8	944-1008 bp	1.0	53.3