Hotspot

rbcL-accD

LSC · 56800-57796 · 997 bp

Back to Adenocalymma

Pi 0.0532

MarkerSeek score 82.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 997
variable_sites: 174
pi: 0.0532
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CGATGCGAAGACAGAGGAAA	GGTTGCTAAGCCTGCGATAT	50.0	50.0	58.0	58.1	306-1146 bp	0.833333	84.4
rbcL-accD_p2	CGATGCGAAGACAGAGGAAA	GATTTCATGTTCCCCGGTCA	50.0	50.0	58.0	57.9	363-388 bp	0.416667	61.8
rbcL-accD_p3	CGATGCGAAGACAGAGGAAA	TGATTTCATGTTCCCCGGTC	50.0	50.0	58.0	57.9	364-389 bp	0.416667	61.8
rbcL-accD_p4	TGTTCGATGCGAAGACAGAG	GGTTGCTAAGCCTGCGATAT	50.0	50.0	58.0	58.1	416-441 bp	0.416667	61.8
rbcL-accD_p5	TGTTCGATGCGAAGACAGAG	GATTTCATGTTCCCCGGTCA	50.0	50.0	58.0	57.9	367-392 bp	0.416667	61.6