primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	rbcL-accD	1	CGATGCGAAGACAGAGGAAA	GGTTGCTAAGCCTGCGATAT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.119473	57380	57499	306-1146	0.833333	84.4
rbcL-accD_p2	rbcL-accD	2	CGATGCGAAGACAGAGGAAA	GATTTCATGTTCCCCGGTCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.126492	57380	57499	363-388	0.416667	61.8
rbcL-accD_p3	rbcL-accD	3	CGATGCGAAGACAGAGGAAA	TGATTTCATGTTCCCCGGTC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.126492	57380	57499	364-389	0.416667	61.8
rbcL-accD_p4	rbcL-accD	4	TGTTCGATGCGAAGACAGAG	GGTTGCTAAGCCTGCGATAT	20	20	50.0	50.0	58.0	58.1	2.1	0.0	0.133153	57380	57499	416-441	0.416667	61.8
rbcL-accD_p5	rbcL-accD	5	TGTTCGATGCGAAGACAGAG	GATTTCATGTTCCCCGGTCA	20	20	50.0	50.0	58.0	57.9	2.1	0.0	0.140171	57380	57499	367-392	0.416667	61.6
accD_p1	accD	1	CGATGCGAAGACAGAGGAAA	CAGGCATAAGCGCTTTTTCC	20	20	50.0	50.0	58.0	58.1	0.0	30.1	0.090392	57797	59521	2623-2982	0.666667	77.9
accD_p2	accD	2	CGATGCGAAGACAGAGGAAA	GCGCCCCTGATCTATTTGAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.036390	57797	59521	2680-2972	0.333333	65.0
accD_p3	accD	3	TGTTCGATGCGAAGACAGAG	GCGCCCCTGATCTATTTGAA	20	20	50.0	50.0	58.0	58.0	2.1	0.0	0.050069	57797	59521	2684-2961	0.250000	61.6
accD_p4	accD	4	CGATGCGAAGACAGAGGAAA	AAGAAACCGTAAAGGCCCAG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.098071	57797	59521	2984-2984	0.083333	42.5
accD_p5	accD	5	CGATGCGAAGACAGAGGAAA	GAAACCGTAAAGGCCCAGAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.103463	57797	59521	2982-2982	0.083333	42.5
ycf4_p1	ycf4	1	TTCAAATAGATCAGGGGCGC	TGGCTCGACTGAATGTTCTG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.117291	60081	60656	910-990	0.833333	84.4
ycf4_p2	ycf4	2	GGAAAAAGCGCTTATGCCTG	TGCATGGATTTGGATACGCA	20	20	50.0	45.0	58.1	58.0	25.3	0.0	0.136572	60081	60656	995-1123	0.833333	84.2
ycf4_p3	ycf4	3	TTCAAATAGATCAGGGGCGC	TGCATGGATTTGGATACGCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.082570	60081	60656	992-1066	0.750000	81.3
ycf4_p4	ycf4	4	TTCAAATAGATCAGGGGCGC	ACAAGACAGAAATGGGCCAG	20	20	50.0	50.0	58.0	58.1	0.0	13.8	0.117541	60081	60656	1242-1344	0.750000	81.1
ycf4_p5	ycf4	5	TTCAAATAGATCAGGGGCGC	CAAGACAGAAATGGGCCAGT	20	20	50.0	50.0	58.0	58.1	0.0	13.8	0.117541	60081	60656	1241-1343	0.750000	81.1
ycf4-cemA_p1	ycf4-cemA	1	TCGTGATGCTTTTCTACGGG	GGAATTCCTGAGCCCAAGAG	20	20	50.0	55.0	58.0	58.0	0.0	17.7	0.054471	60657	61536	1609-1700	0.833333	84.2
ycf4-cemA_p2	ycf4-cemA	2	CGTGATGCTTTTCTACGGGA	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.081227	60657	61536	1277-1348	0.750000	80.0
ycf4-cemA_p3	ycf4-cemA	3	CGTGATGCTTTTCTACGGGA	GGAATTCCTGAGCCCAAGAG	20	20	50.0	55.0	58.0	58.0	0.0	17.7	0.054471	60657	61536	1628-1699	0.750000	78.7
ycf4-cemA_p4	ycf4-cemA	4	TTCTTCTGGTGACCCGTACT	GGAATTCCTGAGCCCAAGAG	20	20	50.0	55.0	58.0	58.0	0.0	17.7	0.055259	60657	61536	1395-1427	0.416667	59.9
ycf4-cemA_p5	ycf4-cemA	5	TAGTGGTCGGAACTTCCAGT	GGAATTCCTGAGCCCAAGAG	20	20	50.0	55.0	58.0	58.0	8.6	17.7	0.055259	60657	61536	1753-1763	0.166667	46.6
rpoA_p1	rpoA	1	ACGTGTGTATCTAGGGAGGG	CCGAGAACTGGTTCACGTAG	20	20	55.0	55.0	57.9	58.0	0.0	23.4	0.090292	78157	79209	1678-1784	1.000000	91.3
rpoA_p2	rpoA	2	ACGTGTGTATCTAGGGAGGG	CGAGAACTGGTTCACGTAGG	20	20	55.0	55.0	57.9	58.0	0.0	19.5	0.090292	78157	79209	1677-1783	1.000000	91.3
rpoA_p3	rpoA	3	ACGTGTGTATCTAGGGAGGG	GGAAGAGACGCAGCATTACA	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.078979	78157	79209	1348-1430	0.916667	88.0
rpoA_p4	rpoA	4	ACGTGTGTATCTAGGGAGGG	CGCTCAAACTGCAACAGAAG	20	20	55.0	50.0	57.9	58.0	0.0	0.8	0.097121	78157	79209	1449-1518	0.916667	87.9
rpoA_p5	rpoA	5	ACGTGTGTATCTAGGGAGGG	AGGGTACGAGAAAGGGAACA	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.088642	78157	79209	1478-1546	0.583333	74.6
rrn16_p1	rrn16	1	TCGAATCCGCTTTGTCTACG	AACTCAGACGTAGCTCCCTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.169748	100298	101788	1621-1627	0.916667	76.6
rrn16_p2	rrn16	2	CGAATCCGCTTTGTCTACGA	AACTCAGACGTAGCTCCCTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.169748	100298	101788	1620-1626	0.916667	76.5
rrn16_p3	rrn16	3	TCGAATCCGCTTTGTCTACG	TGTGAAGCAGTGTCAAACCA	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.217147	100298	101788	1593-1594	0.916667	75.9
rrn16_p4	rrn16	4	CGAATCCGCTTTGTCTACGA	TGTGAAGCAGTGTCAAACCA	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.217147	100298	101788	1592-1593	0.916667	75.8
rrn16_p5	rrn16	5	CGAATCCGCTTTGTCTACGA	TTTTGGGTGTGAAGCAGTGT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.249849	100298	101788	1599-1599	0.833333	72.4
trnI-GAU_p1	trnI-GAU	1	TCTCTCGCTTTTGGCATAGC	TCTATTCTTTTCCCTGGCGC	20	20	50.0	50.0	58.3	58.0	11.0	0.0	0.381735	102088	103100	1083-1084	0.916667	74.1
trnI-GAU_p2	trnI-GAU	2	TCTCTCGCTTTTGGCATAGC	AAGTGGAGCATGCATGAAGT	20	20	50.0	45.0	58.3	57.8	11.0	30.6	0.554598	102088	103100	1117-1118	0.916667	72.7
trnI-GAU_p3	trnI-GAU	3	TCTCTCGCTTTTGGCATAGC	CTATTCTTTTCCCTGGCGCA	20	20	50.0	50.0	58.3	58.2	11.0	10.8	0.593155	102088	103100	1082-1083	0.916667	72.4
trnI-GAU_p4	trnI-GAU	4	TCTCTCGCTTTTGGCATAGC	TATTCTTTTCCCTGGCGCAG	20	20	50.0	50.0	58.3	58.2	11.0	10.8	0.593155	102088	103100	1081-1082	0.916667	72.4
trnI-GAU_p5	trnI-GAU	5	TCTCTCGCTTTTGGCATAGC	CAACGACCCAATTGCAAGAG	20	20	50.0	50.0	58.3	57.7	11.0	4.9	0.679902	102088	103100	1173-1174	0.916667	71.6
trnA-UGC_p1	trnA-UGC	1	TGATTTACTTCACGGGCGAG	AATCATAGATCGAACCGCCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.070575	103165	104039	1061-1070	0.916667	76.8
trnA-UGC_p2	trnA-UGC	2	TGATTTACTTCACGGGCGAG	CATAGATCGAACCGCCGAAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.070575	103165	104039	1058-1067	0.916667	76.8
trnA-UGC_p3	trnA-UGC	3	GCGCCAGGGAAAAGAATAGA	AATCATAGATCGAACCGCCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.104380	103165	104039	1010-1019	0.916667	76.5
trnA-UGC_p4	trnA-UGC	4	GCGCCAGGGAAAAGAATAGA	CATAGATCGAACCGCCGAAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.104380	103165	104039	1007-1016	0.916667	76.5
trnA-UGC_p5	trnA-UGC	5	AGGTCTCTGGTTCAAGTCCA	CATAGATCGAACCGCCGAAT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.169146	103165	104039	1040-1049	0.916667	76.0
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	GGGGTTCGAATCCCTCCT	CCGTTGTTCCTCAGTAGCTC	18	20	61.1	55.0	57.9	58.0	35.1	0.0	2.070588	107885	108370	590-590	0.916667	65.1
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	GGGGTTCGAATCCCTCCT	TGGTCGTAGGTTCGAATCCT	18	20	61.1	50.0	57.9	58.2	35.1	18.9	2.224889	107885	108370	541-541	0.916667	64.3
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	GGGGTTCGAATCCCTCCT	CGATTGGTCGTAGGTTCGAA	18	20	61.1	50.0	57.9	57.7	35.1	0.0	2.335445	107885	108370	545-545	0.916667	63.4
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	GGGGTTCGAATCCCTCCTC	CCGTTGTTCCTCAGTAGCTC	19	20	63.2	55.0	59.2	58.0	35.1	0.0	2.171495	107885	108370	590-590	0.833333	58.9
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	GGGGTTCGAATCCCTCCTC	TGGTCGTAGGTTCGAATCCT	19	20	63.2	50.0	59.2	58.2	35.1	18.9	2.325796	107885	108370	541-541	0.833333	58.0
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	GTGTGGGTCTTCAGCGTATT	ATGAACACCATGAGCTGTCC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.214563	108444	109606	1203-2395	0.750000	80.3
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	TTAACGGTCAAAGTGAGCCC	ATGAACACCATGAGCTGTCC	20	20	50.0	50.0	58.1	57.9	2.1	0.0	0.232915	108444	109606	858-2050	0.750000	80.1
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	GGTAAACGCGCTTTCACATC	ATGAACACCATGAGCTGTCC	20	20	50.0	50.0	58.1	57.9	5.7	0.0	0.241428	108444	109606	1464-2657	0.750000	80.1
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	TCTTAACCCGAAATGGCTGG	ATGAACACCATGAGCTGTCC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.242585	108444	109606	1443-2636	0.750000	80.1
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	TGAACTCTCGGGAGAATCGA	ATGAACACCATGAGCTGTCC	20	20	50.0	50.0	57.9	57.9	21.8	0.0	0.244078	108444	109606	1058-2250	0.750000	80.0
ycf1_p1	ycf1	1	TACTTCTCCGAGCTGAGCTT	TCCGCCTTTTCTTCCGAAAA	20	20	50.0	45.0	58.2	58.0	9.0	0.0	0.189171	110121	110240	817-823	0.750000	74.6
ycf1_p2	ycf1	2	TTACTTCTCCGAGCTGAGCT	TCCGCCTTTTCTTCCGAAAA	20	20	50.0	45.0	58.2	58.0	6.7	0.0	0.189171	110121	110240	818-824	0.750000	74.5
ycf1_p3	ycf1	3	ACTTCTCCGAGCTGAGCTTA	TCCGCCTTTTCTTCCGAAAA	20	20	50.0	45.0	58.2	58.0	9.0	0.0	0.189171	110121	110240	816-816	0.583333	66.6
ycf1_p4	ycf1	4	ACTTCTCCGAGCTGAGCTTA	CACTTTTGGGGGTTCTTCCA	20	20	50.0	50.0	58.2	57.9	9.0	0.0	0.239917	110121	110240	679-679	0.500000	61.3
ycf1_p5	ycf1	5	GGCAGGCCTCATACGATAAC	TCCGCCTTTTCTTCCGAAAA	20	20	55.0	45.0	58.2	58.0	0.0	0.0	0.232884	110121	110240	692-692	0.250000	49.1
rpl32-trnL-UAC_p1	rpl32-trnL-UAC	1	TTTTTGTGCGACAAACAAAT	CTCGCACTGCTTCCTAAGAG	20	20	30.0	55.0	53.4	58.1	12.1	0.0	4.703638	118608	119485	957-1021	1.000000	54.2
rpl32-trnL-UAC_p2	rpl32-trnL-UAC	2	TTTTTGTGCGACAAACAAAT	CTCTCGCACTGCTTCCTAAG	20	20	30.0	55.0	53.4	58.1	12.1	0.0	4.703638	118608	119485	959-1023	1.000000	54.2
rpl32-trnL-UAC_p3	rpl32-trnL-UAC	3	TTTTTGTGCGACAAACAAAT	TCCTAAGAGCAGCGTGTCTA	20	20	30.0	50.0	53.4	57.9	12.1	0.0	4.747833	118608	119485	946-1010	1.000000	53.9
rpl32-trnL-UAC_p4	rpl32-trnL-UAC	4	TTTTTGTGCGACAAACAAAT	AGCAGCGTGTCTACCAATTT	20	20	30.0	45.0	53.4	57.8	12.1	0.0	4.808560	118608	119485	939-1003	1.000000	53.4
rpl32-trnL-UAC_p5	rpl32-trnL-UAC	5	TTTTTGTGCGACAAACAAAT	CTAAGAGCAGCGTGTCTACC	20	20	30.0	55.0	53.4	57.8	12.1	0.0	4.819851	118608	119485	944-1008	1.000000	53.3
ccsA-ndhD_p1	ccsA-ndhD	1	GAAGCATGGGGGTCCTATTG	TTTTGATTCGGGACCACGAG	20	20	55.0	50.0	58.0	58.2	0.0	5.4	0.209784	120623	120854	602-616	1.000000	90.3
ccsA-ndhD_p2	ccsA-ndhD	2	GAAGCATGGGGGTCCTATTG	ATGGCTTCCCTTGCATTACC	20	20	55.0	50.0	58.0	58.2	0.0	0.0	0.232615	120623	120854	817-831	1.000000	88.5
ccsA-ndhD_p3	ccsA-ndhD	3	GAAGCATGGGGGTCCTATTG	CTCAACGGAGCCCTTTTACA	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.192030	120623	120854	982-996	1.000000	87.7
ccsA-ndhD_p4	ccsA-ndhD	4	GAAGCATGGGGGTCCTATTG	TGATGGTAGGTGCAATGCAA	20	20	55.0	45.0	58.0	57.8	0.0	10.4	0.225930	120623	120854	1122-1136	1.000000	86.7
ccsA-ndhD_p5	ccsA-ndhD	5	CGGCCCTTTTATGTGGATCA	CTCAACGGAGCCCTTTTACA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.230246	120623	120854	1281-1295	1.000000	86.3
ndhD-psaC_p1	ndhD-psaC	1	GAACCCGCAAATATTGGCAA	TGTGAATCTGCCTGTCCAAC	20	20	45.0	50.0	57.3	58.1	15.1	0.0	0.767607	122364	122495	263-276	1.000000	84.9
ndhD-psaC_p2	ndhD-psaC	2	AGGAACCCGCAAATATTGGC	TGTGAATCTGCCTGTCCAAC	20	20	50.0	50.0	58.9	58.1	4.6	0.0	0.992503	122364	122495	265-278	1.000000	83.4
ndhD-psaC_p3	ndhD-psaC	3	GAACCCGCAAATATTGGCAA	GATGTGAATCTGCCTGTCCA	20	20	45.0	50.0	57.3	57.6	15.1	0.0	1.072097	122364	122495	265-278	1.000000	82.4
ndhD-psaC_p4	ndhD-psaC	4	CCCGCAAATATTGGCAAAACT	TGTGAATCTGCCTGTCCAAC	21	20	42.9	50.0	58.0	58.1	15.1	0.0	1.132473	122364	122495	260-273	1.000000	82.1
ndhD-psaC_p5	ndhD-psaC	5	GAACCCGCAAATATTGGCAA	GCCTGTCCAACGGATTTCTT	20	20	45.0	50.0	57.3	58.5	15.1	0.0	1.129098	122364	122495	254-267	0.916667	78.6
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	ATGAACACCATGAGCTGTCC	GTGTGGGTCTTCAGCGTATT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.214563	134472	135634	1203-2395	0.833333	83.6
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	ATGAACACCATGAGCTGTCC	TTAACGGTCAAAGTGAGCCC	20	20	50.0	50.0	57.9	58.1	0.0	2.1	0.232915	134472	135634	858-2050	0.833333	83.5
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	ATGAACACCATGAGCTGTCC	GGTAAACGCGCTTTCACATC	20	20	50.0	50.0	57.9	58.1	0.0	5.7	0.241428	134472	135634	1464-2657	0.833333	83.4
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	ATGAACACCATGAGCTGTCC	TCTTAACCCGAAATGGCTGG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.242585	134472	135634	1443-2636	0.833333	83.4
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	ATGAACACCATGAGCTGTCC	TGAACTCTCGGGAGAATCGA	20	20	50.0	50.0	57.9	57.9	0.0	21.8	0.244078	134472	135634	1058-2250	0.833333	83.4