Hotspot

clpP

LSC · 74256-76418 · 2163 bp

Back to Adansonia

Pi 0.00586

MarkerSeek score 60.40

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2163
variable_sites: 36
pi: 0.00586
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGGATTGGTGTTAAATCGTTGC	ACGCTTCCACATCAAAGTGA	40.9	45.0	57.8	57.7	2227-2250 bp	0.888889	58.8
clpP_p2	GGTGTTAAATCGTTGCTATTGCT	ACGCTTCCACATCAAAGTGA	39.1	45.0	58.0	57.7	2221-2244 bp	1.0	56.9
clpP_p3	GGATTGGTGTTAAATCGTTGCT	ACGCTTCCACATCAAAGTGA	40.9	45.0	57.6	57.7	2226-2249 bp	0.888889	56.7
clpP_p4	GGATTGGTGTTAAATCGTTGC	ACGCTTCCACATCAAAGTGA	42.9	45.0	56.1	57.7	2226-2249 bp	0.888889	53.2
clpP_p5	TGTGGATTGGTGTTAAATCGTTG	ACGCTTCCACATCAAAGTGA	39.1	45.0	57.9	57.7	2229-2252 bp	0.888889	51.2