Hotspot

rpl32-trnL-UAG

SSC · 118194-119039 · 846 bp

Back to Adansonia

Pi 0.00709
MarkerSeek score 56.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
846
variable_sites
18
pi
0.00709
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCGTTGAAAGCTTTGTCAT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.6 58.0 953-965 bp 1.0 79.5
rpl32-trnL-UAG_p2 GGCGTTGAAAGCTTTGTCAT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.6 58.0 954-966 bp 1.0 79.5
rpl32-trnL-UAG_p3 GGCGTTGAAAGCTTTGTCAT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.6 57.9 963-975 bp 1.0 78.5
rpl32-trnL-UAG_p4 GGCGTTGAAAGCTTTGTCAT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.6 57.8 962-974 bp 1.0 77.9
rpl32-trnL-UAG_p5 GGCGTTGAAAGCTTTGTCAT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.6 57.8 961-973 bp 1.0 77.9