Hotspot

ycf1-ndhF

SSC · 114521-114587 · 67 bp

Back to Adansonia

Pi 0.0506

MarkerSeek score 79.10

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 67
variable_sites: 10
pi: 0.0506
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	AAAAAGGGAGGTTAATGATGA	GGGGAAGTCGCATCTCTTTT	33.3	50.0	52.7	57.9	191-253 bp	1.0	47.5
ycf1-ndhF_p2	AGGGAGGTTAATGATGATTTT	ATATGTAGGGGGAAGTCGCA	33.3	50.0	52.4	57.9	195-257 bp	1.0	47.5
ycf1-ndhF_p3	AGGGAGGTTAATGATGATTTT	TATGTAGGGGGAAGTCGCAT	33.3	50.0	52.4	57.9	194-256 bp	1.0	47.5
ycf1-ndhF_p4	AAAAAGGGAGGTTAATGATGA	ATATGTAGGGGGAAGTCGCA	33.3	50.0	52.7	57.9	199-261 bp	1.0	47.4
ycf1-ndhF_p5	AAAAAGGGAGGTTAATGATGA	TATGTAGGGGGAAGTCGCAT	33.3	50.0	52.7	57.9	198-260 bp	1.0	47.4