Hotspot

petA-psbJ

LSC · 67143-68199 · 1057 bp

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Pi 0.00426

MarkerSeek score 61.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1057
variable_sites: 12
pi: 0.00426
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTACGTGTCCAAGGCCTTTT	ATTCTGGATTGGGTTCGTCC	50.0	50.0	58.1	57.6	1173-1206 bp	1.0	78.5
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	ATTCTGGATTGGGTTCGTCC	50.0	50.0	57.8	57.6	1161-1194 bp	1.0	78.2
petA-psbJ_p3	GATCCACTACGTGTCCAAGG	ATTCTGGATTGGGTTCGTCC	55.0	50.0	57.7	57.6	1179-1212 bp	1.0	77.0
petA-psbJ_p4	TACGTGTCCAAGGCCTTTTG	ATTCTGGATTGGGTTCGTCC	50.0	50.0	58.4	57.6	1172-1205 bp	1.0	76.2
petA-psbJ_p5	TGTCCAAGGCCTTTTGTTCT	ATTCTGGATTGGGTTCGTCC	45.0	50.0	57.5	57.6	1168-1201 bp	1.0	75.8