Hotspot

accD-psaI

LSC · 62459-63200 · 742 bp

Back to Adansonia

Pi 0.00719

MarkerSeek score 42.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 742
variable_sites: 12
pi: 0.00719
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GAGTTAGTTCAGCTCCACGG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	869-875 bp	1.0	80.9
accD-psaI_p2	AGTTCAGCTCCACGGTTTTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.9	58.0	864-870 bp	1.0	80.5
accD-psaI_p3	GAGTTAGTTCAGCTCCACGG	GAGGGAAAGCTGCTGAAAGT	55.0	50.0	58.0	58.1	822-828 bp	1.0	80.4
accD-psaI_p4	GAGTTAGTTCAGCTCCACGG	AAGAAGCCATTGCAATTGCC	55.0	45.0	58.0	57.9	875-881 bp	1.0	80.2
accD-psaI_p5	AGTTCAGCTCCACGGTTTTT	GAGGGAAAGCTGCTGAAAGT	45.0	50.0	57.9	58.1	817-823 bp	1.0	80.0