Hotspot

atpB-rbcL

LSC · 57781-58812 · 1032 bp

Back to Adansonia

Pi 0.00794

MarkerSeek score 55.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1032
variable_sites: 23
pi: 0.00794
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTCAAGCGTGGAAACTCCA	GGTGACATAAGTCCCTCCCT	45.0	55.0	58.0	58.1	1066-1154 bp	1.0	84.3
atpB-rbcL_p2	TTTCAAGCGTGGAAACTCCA	TGGTGACATAAGTCCCTCCC	45.0	55.0	58.0	58.4	1067-1155 bp	1.0	81.9
atpB-rbcL_p3	TTTCAAGCGTGGAAACTCCA	GTGGTGACATAAGTCCCTCC	45.0	55.0	58.0	57.3	1068-1156 bp	1.0	79.7
atpB-rbcL_p4	TTTCAAGCGTGGAAACTCCA	AAGTCCCTCCCTACAACTCA	45.0	50.0	58.0	57.3	1058-1146 bp	1.0	79.6
atpB-rbcL_p5	TTTCAAGCGTGGAAACTCCA	AGTCCCTCCCTACAACTCAT	45.0	50.0	58.0	57.0	1057-1145 bp	1.0	77.7