Hotspot

atpH-atpI

LSC · 14278-15480 · 1203 bp

Back to Adansonia

Pi 0.0036

MarkerSeek score 54.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1203
variable_sites: 12
pi: 0.0036
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1320-1332 bp	1.0	79.9
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1286-1298 bp	1.0	79.9
atpH-atpI_p3	AATAGAAGCAAGCCCGACAG	CGAATCCATGGAGGGTCATC	50.0	55.0	58.0	57.8	1290-1302 bp	1.0	79.6
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1256-1268 bp	1.0	79.6
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1342-1354 bp	1.0	79.2