Hotspot

trnS-GCU-trnG-GCC

LSC · 8446-9248 · 803 bp

Back to Adansonia

Pi 0.0103

MarkerSeek score 65.00

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 35
pi: 0.0103
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	ATGAATCAAACCGAGGGACC	50.0	50.0	58.0	57.6	736-919 bp	1.0	83.7
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	ATGAATCAAACCGAGGGACC	50.0	50.0	58.0	57.6	735-918 bp	1.0	83.7
trnS-GCU-trnG-GCC_p3	CGCACAACGGATTAGCAATC	ATGAATCAAACCGAGGGACC	50.0	50.0	57.9	57.6	751-934 bp	1.0	83.0
trnS-GCU-trnG-GCC_p4	ACGGAAAGAGAGGGATTCGA	ATGAATCAAACCGAGGGACC	50.0	50.0	58.2	57.6	790-973 bp	1.0	82.4
trnS-GCU-trnG-GCC_p5	TCGCACAACGGATTAGCAAT	ATGAATCAAACCGAGGGACC	45.0	50.0	58.3	57.6	752-935 bp	1.0	81.6