Hotspot

ycf3

LSC · 44711-46684 · 1974 bp

Back to Actinodaphne

Pi 0.00456

MarkerSeek score 56.60

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1974
variable_sites: 17
pi: 0.00456
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TTCCCTGATTGGCTCTTCCT	TGGAATCATTTTCCCCTGAGG	50.0	47.6	58.3	57.6	2115-2119 bp	1.0	67.0
ycf3_p2	TCCCTGATTGGCTCTTCCTA	TGGAATCATTTTCCCCTGAGG	50.0	47.6	57.4	57.6	2114-2118 bp	1.0	65.1
ycf3_p3	ATTCCCTGATTGGCTCTTCC	TGGAATCATTTTCCCCTGAGG	50.0	47.6	57.3	57.6	2116-2120 bp	1.0	63.7
ycf3_p4	TTCCCTGATTGGCTCTTCCT	TGTGGAATCATTTTCCCCTGA	50.0	42.9	58.3	57.2	2117-2121 bp	1.0	63.5
ycf3_p5	CCCTGATTGGCTCTTCCTAG	TGGAATCATTTTCCCCTGAGG	55.0	47.6	57.1	57.6	2113-2117 bp	1.0	62.2