Hotspot

ycf1

SSC · 128423-133972 · 5550 bp

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Pi 0.00763

MarkerSeek score 54.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5550
variable_sites: 78
pi: 0.00763
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGATCAGTTGTAATTACATCGA	AGGCTTCCTACTGATTACCA	34.8	45.0	55.5	55.2	237 bp	1.0	43.0
ycf1_p2	TGGATCAGTTGTAATTACATCGA	AGGCTTCCTACTGATTACCAA	34.8	42.9	55.5	56.1	237 bp	1.0	43.0
ycf1_p3	TGGATCAGTTGTAATTACATCGAA	AGGCTTCCTACTGATTACCA	33.3	45.0	56.2	55.2	237 bp	1.0	43.0
ycf1_p4	TTGGATCAGTTGTAATTACATCGA	AGGCTTCCTACTGATTACCA	33.3	45.0	56.2	55.2	238 bp	1.0	43.0
ycf1_p5	TGGATCAGTTGTAATTACATCGA	AGGCTTCCTACTGATTACCAAA	34.8	40.9	55.5	56.8	237 bp	1.0	43.0