Hotspot

psaB

LSC · 39589-41793 · 2205 bp

Back to Actinodaphne

Pi 0.00204

MarkerSeek score 58.70

Primer available yes

Label psaB

Feature Metadata

label_name: psaB
feature_type: gene
region: LSC
length_bp: 2205
variable_sites: 8
pi: 0.00204
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB_p1	AATGATACGGGTCGGATCCT	CCACAACATGGGCATTCTTC	50.0	50.0	58.0	57.3	2381-2384 bp	1.0	74.9
psaB_p2	CAATGATACGGGTCGGATCC	CCACAACATGGGCATTCTTC	55.0	50.0	57.9	57.3	2382-2385 bp	1.0	74.3
psaB_p3	AGGTGGATTCTCCCTTCTCC	CCACAACATGGGCATTCTTC	55.0	50.0	58.1	57.3	2343-2346 bp	1.0	74.1
psaB_p4	CGGGTCGGATCCTAGATGTA	CCACAACATGGGCATTCTTC	55.0	50.0	57.8	57.3	2374-2377 bp	1.0	73.4
psaB_p5	TACGGGTCGGATCCTAGATG	CCACAACATGGGCATTCTTC	55.0	50.0	57.8	57.3	2376-2379 bp	1.0	73.4