Hotspot

ndhF

SSC · 113817-116045 · 2229 bp

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Pi 0.00912

MarkerSeek score 48.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 37
pi: 0.00912
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	CCGGAAGAAATAAGGGCTGG	50.0	55.0	58.0	58.3	2859-2943 bp	1.0	78.4
ndhF_p2	AAGAGGGATCCACCGAAGAA	CCGGAAGAAATAAGGGCTGG	50.0	55.0	58.0	58.3	2851-2935 bp	1.0	78.4
ndhF_p3	ACAGGAACAAGAGGGATCCA	ATAAGGGCTGGTGAATGGGA	50.0	50.0	58.0	58.4	2850-2934 bp	1.0	77.8
ndhF_p4	AAGAGGGATCCACCGAAGAA	ATAAGGGCTGGTGAATGGGA	50.0	50.0	58.0	58.4	2842-2926 bp	1.0	77.8
ndhF_p5	TTTAGGCAGAATACCGTCGC	CCGGAAGAAATAAGGGCTGG	50.0	55.0	58.1	58.3	2988-2997 bp	0.75	68.2