Hotspot

atpF

LSC · 13039-14323 · 1285 bp

Back to Actinodaphne

Pi 0.00415

MarkerSeek score 62.10

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1285
variable_sites: 10
pi: 0.00415
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCGGCTCGAA	GGGGGCGAAGTGATACAAAA	45.0	50.0	57.4	58.2	1445-1447 bp	1.0	74.4
atpF_p2	ACTAATTTCGTCGGCTCGAA	CAATCCAAAACAAAGGGGGC	45.0	50.0	57.4	57.8	1459-1461 bp	1.0	74.3
atpF_p3	AATTTCGTCGGCTCGAATGG	GGGGGCGAAGTGATACAAAA	50.0	50.0	59.0	58.2	1442-1444 bp	1.0	71.6
atpF_p4	AATTTCGTCGGCTCGAATGG	CAATCCAAAACAAAGGGGGC	50.0	50.0	59.0	57.8	1456-1458 bp	1.0	71.5
atpF_p5	ACTAATTTCGTCGGCTCGAA	CAAAGGGGGCGAAGTGATAC	45.0	55.0	57.4	58.6	1449-1451 bp	1.0	70.8