Hotspot

ndhF-rpl32

SSC · 116046-117168 · 1123 bp

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Pi 0.0188

MarkerSeek score 59.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1123
variable_sites: 39
pi: 0.0188
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAACAAGCAGAAGTCCCAA	TACGAATCTTATCCCCGCCT	50.0	50.0	58.0	58.0	1842-1858 bp	1.0	83.0
ndhF-rpl32_p2	AGCAGAAGTCCCAACCCTAT	TACGAATCTTATCCCCGCCT	50.0	50.0	58.0	58.0	1836-1852 bp	1.0	83.0
ndhF-rpl32_p3	AATAGGGAGGCACACAGAGT	TACGAATCTTATCCCCGCCT	50.0	50.0	58.0	58.0	2451-2467 bp	1.0	82.3
ndhF-rpl32_p4	AAGGGGAAATTGTGCGGATT	TACGAATCTTATCCCCGCCT	45.0	50.0	58.1	58.0	2491-2507 bp	1.0	82.1
ndhF-rpl32_p5	AGGGGAAATTGTGCGGATTT	TACGAATCTTATCCCCGCCT	45.0	50.0	58.1	58.0	2490-2506 bp	1.0	82.1