Hotspot

ycf1-ndhF

SSC · 113799-113816 · 18 bp

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Pi 0.0926

MarkerSeek score 77.20

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 18
variable_sites: 3
pi: 0.0926
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACAGGAACAAGAGGGATCCA	CAATGGTGTTGGTGTTGCAA	50.0	45.0	58.0	57.7	710-794 bp	1.0	78.4
ycf1-ndhF_p2	AAGAGGGATCCACCGAAGAA	CAATGGTGTTGGTGTTGCAA	50.0	45.0	58.0	57.7	702-786 bp	1.0	78.4
ycf1-ndhF_p3	TTTAGGCAGAATACCGTCGC	CAATGGTGTTGGTGTTGCAA	50.0	45.0	58.1	57.7	839-923 bp	1.0	78.1
ycf1-ndhF_p4	ACAGGAACAAGAGGGATCCA	GGTGTTGGTGTTGCAAGTTT	50.0	45.0	58.0	57.6	706-790 bp	1.0	77.7
ycf1-ndhF_p5	AAGAGGGATCCACCGAAGAA	GGTGTTGGTGTTGCAAGTTT	50.0	45.0	58.0	57.6	698-782 bp	1.0	77.7