Hotspot

rpl2-rpl23

LSC · 89405-89555 · 151 bp

Back to Actinodaphne

Pi 0.0143

MarkerSeek score 62.20

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: LSC
length_bp: 151
variable_sites: 4
pi: 0.0143
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	TTACGACCTTTACCACAGCG	CATCAACTACCCGGGAAAGG	50.0	55.0	57.9	58.0	292-296 bp	1.0	80.8
rpl2-rpl23_p2	TCGCGGATTGGATTTCACTT	CATCAACTACCCGGGAAAGG	45.0	55.0	57.9	58.0	248-252 bp	1.0	80.8
rpl2-rpl23_p3	TTCGCGGATTGGATTTCACT	CATCAACTACCCGGGAAAGG	45.0	55.0	57.9	58.0	249-253 bp	1.0	80.7
rpl2-rpl23_p4	TTACGACCTTTACCACAGCG	AGTCATCAACTACCCGGGAA	50.0	50.0	57.9	58.1	295-299 bp	1.0	80.6
rpl2-rpl23_p5	TCGCGGATTGGATTTCACTT	AGTCATCAACTACCCGGGAA	45.0	50.0	57.9	58.1	251-255 bp	1.0	80.6