Hotspot

rpl20-rps12

LSC · 72601-73356 · 756 bp

Back to Actinodaphne

Pi 0.00463

MarkerSeek score 62.10

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 6
pi: 0.00463
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	910-914 bp	1.0	75.5
rpl20-rps12_p2	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	909-913 bp	1.0	75.5
rpl20-rps12_p3	CTTGAATGAGCCCCTCGAAA	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	873-877 bp	1.0	74.0
rpl20-rps12_p4	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	872-876 bp	1.0	74.0
rpl20-rps12_p5	TTTGTTCTACGTCTCCGAGC	AAATGTCACGAAATCCCCCG	50.0	50.0	57.7	58.5	868-872 bp	1.0	73.7