Hotspot

petA-psbJ

LSC · 66414-67556 · 1143 bp

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Pi 0.0151

MarkerSeek score 65.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1143
variable_sites: 33
pi: 0.0151
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTTTGTTGT	GGCTCATATTCCGGATTGGG	45.0	55.0	58.2	58.2	1280-1288 bp	1.0	79.9
petA-psbJ_p2	ACGTGTCCAAGGTTTGTTGT	CGGCTCATATTCCGGATTGG	45.0	55.0	58.2	58.6	1281-1289 bp	1.0	76.8
petA-psbJ_p3	ACGTGTCCAAGGTTTGTTGT	GCTCATATTCCGGATTGGGA	45.0	50.0	58.2	57.1	1279-1287 bp	1.0	74.4
petA-psbJ_p4	ACGTGTCCAAGGTTTGTTGT	ATTCCGGATTGGGATCATCC	45.0	50.0	58.2	57.1	1273-1281 bp	1.0	74.2
petA-psbJ_p5	CGTGTCCAAGGTTTGTTGTT	GGCTCATATTCCGGATTGGG	45.0	55.0	57.1	58.2	1279-1287 bp	1.0	73.8