primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	atpF	1	ACTAATTTCGTCGGCTCGAA	GGGGGCGAAGTGATACAAAA	20	20	45.0	50.0	57.4	58.2	9.6	0.0	0.813159	13039	14323	1445-1447	1.000000	74.4
atpF_p2	atpF	2	ACTAATTTCGTCGGCTCGAA	CAATCCAAAACAAAGGGGGC	20	20	45.0	50.0	57.4	57.8	9.6	0.0	0.825617	13039	14323	1459-1461	1.000000	74.3
atpF_p3	atpF	3	AATTTCGTCGGCTCGAATGG	GGGGGCGAAGTGATACAAAA	20	20	50.0	50.0	59.0	58.2	13.2	0.0	1.164188	13039	14323	1442-1444	1.000000	71.6
atpF_p4	atpF	4	AATTTCGTCGGCTCGAATGG	CAATCCAAAACAAAGGGGGC	20	20	50.0	50.0	59.0	57.8	13.2	0.0	1.176646	13039	14323	1456-1458	1.000000	71.5
atpF_p5	atpF	5	ACTAATTTCGTCGGCTCGAA	CAAAGGGGGCGAAGTGATAC	20	20	45.0	55.0	57.4	58.6	9.6	0.0	1.262612	13039	14323	1449-1451	1.000000	70.8
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTCCTTAACC	20	20	55.0	55.0	58.1	57.9	7.6	0.0	0.169074	33739	35179	1583-1588	1.000000	80.0
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTCCTTAACC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.191228	33739	35179	1586-1591	1.000000	79.8
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	33739	35179	1591-1596	1.000000	78.8
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	33739	35179	1594-1599	1.000000	78.6
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GTGGTAGAGTAACGCCATGG	CGAAACGGTCCCTCCTTAAC	20	20	55.0	55.0	58.1	58.3	7.6	0.0	0.345672	33739	35179	1584-1589	1.000000	78.5
psaB_p1	psaB	1	AATGATACGGGTCGGATCCT	CCACAACATGGGCATTCTTC	20	20	50.0	50.0	58.0	57.3	10.8	0.0	0.671311	39589	41793	2381-2384	1.000000	74.9
psaB_p2	psaB	2	CAATGATACGGGTCGGATCC	CCACAACATGGGCATTCTTC	20	20	55.0	50.0	57.9	57.3	0.0	0.0	0.740475	39589	41793	2382-2385	1.000000	74.3
psaB_p3	psaB	3	AGGTGGATTCTCCCTTCTCC	CCACAACATGGGCATTCTTC	20	20	55.0	50.0	58.1	57.3	0.0	0.0	0.773766	39589	41793	2343-2346	1.000000	74.1
psaB_p4	psaB	4	CGGGTCGGATCCTAGATGTA	CCACAACATGGGCATTCTTC	20	20	55.0	50.0	57.8	57.3	10.8	0.0	0.855930	39589	41793	2374-2377	1.000000	73.4
psaB_p5	psaB	5	TACGGGTCGGATCCTAGATG	CCACAACATGGGCATTCTTC	20	20	55.0	50.0	57.8	57.3	10.8	0.0	0.855930	39589	41793	2376-2379	1.000000	73.4
ycf3_p1	ycf3	1	TTCCCTGATTGGCTCTTCCT	TGGAATCATTTTCCCCTGAGG	20	21	50.0	47.6	58.3	57.6	0.0	0.0	1.713165	44711	46684	2115-2119	1.000000	67.0
ycf3_p2	ycf3	2	TCCCTGATTGGCTCTTCCTA	TGGAATCATTTTCCCCTGAGG	20	21	50.0	47.6	57.4	57.6	0.0	0.0	1.955478	44711	46684	2114-2118	1.000000	65.1
ycf3_p3	ycf3	3	ATTCCCTGATTGGCTCTTCC	TGGAATCATTTTCCCCTGAGG	20	21	50.0	47.6	57.3	57.6	0.0	0.0	2.131284	44711	46684	2116-2120	1.000000	63.7
ycf3_p4	ycf3	4	TTCCCTGATTGGCTCTTCCT	TGTGGAATCATTTTCCCCTGA	20	21	50.0	42.9	58.3	57.2	0.0	2.1	2.150234	44711	46684	2117-2121	1.000000	63.5
ycf3_p5	ycf3	5	CCCTGATTGGCTCTTCCTAG	TGGAATCATTTTCCCCTGAGG	20	21	55.0	47.6	57.1	57.6	0.0	0.0	2.313710	44711	46684	2113-2117	1.000000	62.2
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	48886	49444	589-609	1.000000	82.8
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	48886	49444	597-617	1.000000	82.2
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	AATCCAACCAAGTCCGTAGC	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.242165	48886	49444	613-633	1.000000	81.8
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	48886	49444	621-641	1.000000	81.7
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	48886	49444	629-649	1.000000	81.1
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	20	20	55.0	50.0	58.0	58.1	10.3	0.0	0.066573	50429	50995	723-741	1.000000	82.2
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	TCCTCTAATAGGGCGATGCA	ATCCACGCCTGAAACGTATC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.073749	50429	50995	755-773	1.000000	82.0
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.115963	50429	50995	687-705	1.000000	81.9
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTGAAACGTATC	20	20	55.0	50.0	57.9	58.1	2.5	0.0	0.200366	50429	50995	711-729	1.000000	81.2
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	TGACATAGACCCCAGTCCTC	ATCCACGCCTGAAACGTATC	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.224788	50429	50995	770-788	1.000000	80.8
petA-psbJ_p1	petA-psbJ	1	ACGTGTCCAAGGTTTGTTGT	GGCTCATATTCCGGATTGGG	20	20	45.0	55.0	58.2	58.2	0.0	4.1	0.344636	66414	67556	1280-1288	1.000000	79.9
petA-psbJ_p2	petA-psbJ	2	ACGTGTCCAAGGTTTGTTGT	CGGCTCATATTCCGGATTGG	20	20	45.0	55.0	58.2	58.6	0.0	4.1	0.728252	66414	67556	1281-1289	1.000000	76.8
petA-psbJ_p3	petA-psbJ	3	ACGTGTCCAAGGTTTGTTGT	GCTCATATTCCGGATTGGGA	20	20	45.0	50.0	58.2	57.1	0.0	4.1	1.030186	66414	67556	1279-1287	1.000000	74.4
petA-psbJ_p4	petA-psbJ	4	ACGTGTCCAAGGTTTGTTGT	ATTCCGGATTGGGATCATCC	20	20	45.0	50.0	58.2	57.1	0.0	4.1	1.058172	66414	67556	1273-1281	1.000000	74.2
petA-psbJ_p5	petA-psbJ	5	CGTGTCCAAGGTTTGTTGTT	GGCTCATATTCCGGATTGGG	20	20	45.0	55.0	57.1	58.2	0.0	4.1	1.105127	66414	67556	1279-1287	1.000000	73.8
rpl20-rps12_p1	rpl20-rps12	1	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.654410	72601	73356	910-914	1.000000	75.5
rpl20-rps12_p2	rpl20-rps12	2	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.654410	72601	73356	909-913	1.000000	75.5
rpl20-rps12_p3	rpl20-rps12	3	CTTGAATGAGCCCCTCGAAA	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	57.9	58.7	0.0	9.8	0.844694	72601	73356	873-877	1.000000	74.0
rpl20-rps12_p4	rpl20-rps12	4	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	57.9	58.7	0.0	9.8	0.844694	72601	73356	872-876	1.000000	74.0
rpl20-rps12_p5	rpl20-rps12	5	TTTGTTCTACGTCTCCGAGC	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.7	58.5	0.0	0.0	0.883460	72601	73356	868-872	1.000000	73.7
rpl2-rpl23_p1	rpl2-rpl23	1	TTACGACCTTTACCACAGCG	CATCAACTACCCGGGAAAGG	20	20	50.0	55.0	57.9	58.0	0.0	32.0	0.107224	89405	89555	292-296	1.000000	80.8
rpl2-rpl23_p2	rpl2-rpl23	2	TCGCGGATTGGATTTCACTT	CATCAACTACCCGGGAAAGG	20	20	45.0	55.0	57.9	58.0	6.1	32.0	0.146337	89405	89555	248-252	1.000000	80.8
rpl2-rpl23_p3	rpl2-rpl23	3	TTCGCGGATTGGATTTCACT	CATCAACTACCCGGGAAAGG	20	20	45.0	55.0	57.9	58.0	6.1	32.0	0.146337	89405	89555	249-253	1.000000	80.7
rpl2-rpl23_p4	rpl2-rpl23	4	TTACGACCTTTACCACAGCG	AGTCATCAACTACCCGGGAA	20	20	50.0	50.0	57.9	58.1	0.0	32.0	0.121852	89405	89555	295-299	1.000000	80.6
rpl2-rpl23_p5	rpl2-rpl23	5	TCGCGGATTGGATTTCACTT	AGTCATCAACTACCCGGGAA	20	20	45.0	50.0	57.9	58.1	6.1	32.0	0.160966	89405	89555	251-255	1.000000	80.6
ycf2_p1	ycf2	1	TGAATCATCCCCATCGCTTC	TGCCAGATACCTGTGACTCA	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.323653	93151	93270	213-213	1.000000	79.7
ycf2_p2	ycf2	2	TCTTGAATCATCCCCATCGC	TGCCAGATACCTGTGACTCA	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.323653	93151	93270	216-216	1.000000	79.6
ycf2_p3	ycf2	3	AATCATCCCCATCGCTTCTG	TGCCAGATACCTGTGACTCA	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.329983	93151	93270	211-211	1.000000	79.6
ycf2_p4	ycf2	4	CTTGAATCATCCCCATCGCT	TGCCAGATACCTGTGACTCA	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.329983	93151	93270	215-215	1.000000	79.6
ycf2_p5	ycf2	5	GAATCATCCCCATCGCTTCT	TGCCAGATACCTGTGACTCA	20	20	50.0	50.0	57.4	58.1	0.0	0.0	0.620728	93151	93270	212-212	1.000000	77.3
ycf1-ndhF_p1	ycf1-ndhF	1	ACAGGAACAAGAGGGATCCA	CAATGGTGTTGGTGTTGCAA	20	20	50.0	45.0	58.0	57.7	15.2	0.0	0.351134	113799	113816	710-794	1.000000	78.4
ycf1-ndhF_p2	ycf1-ndhF	2	AAGAGGGATCCACCGAAGAA	CAATGGTGTTGGTGTTGCAA	20	20	50.0	45.0	58.0	57.7	15.2	0.0	0.353720	113799	113816	702-786	1.000000	78.4
ycf1-ndhF_p3	ycf1-ndhF	3	TTTAGGCAGAATACCGTCGC	CAATGGTGTTGGTGTTGCAA	20	20	50.0	45.0	58.1	57.7	0.0	0.0	0.375774	113799	113816	839-923	1.000000	78.1
ycf1-ndhF_p4	ycf1-ndhF	4	ACAGGAACAAGAGGGATCCA	GGTGTTGGTGTTGCAAGTTT	20	20	50.0	45.0	58.0	57.6	15.2	0.0	0.423237	113799	113816	706-790	1.000000	77.7
ycf1-ndhF_p5	ycf1-ndhF	5	AAGAGGGATCCACCGAAGAA	GGTGTTGGTGTTGCAAGTTT	20	20	50.0	45.0	58.0	57.6	15.2	0.0	0.425823	113799	113816	698-782	1.000000	77.7
ndhF_p1	ndhF	1	ACAGGAACAAGAGGGATCCA	CCGGAAGAAATAAGGGCTGG	20	20	50.0	55.0	58.0	58.3	15.2	0.0	0.364455	113817	116045	2859-2943	1.000000	78.4
ndhF_p2	ndhF	2	AAGAGGGATCCACCGAAGAA	CCGGAAGAAATAAGGGCTGG	20	20	50.0	55.0	58.0	58.3	15.2	0.0	0.367041	113817	116045	2851-2935	1.000000	78.4
ndhF_p3	ndhF	3	ACAGGAACAAGAGGGATCCA	ATAAGGGCTGGTGAATGGGA	20	20	50.0	50.0	58.0	58.4	15.2	0.0	0.437568	113817	116045	2850-2934	1.000000	77.8
ndhF_p4	ndhF	4	AAGAGGGATCCACCGAAGAA	ATAAGGGCTGGTGAATGGGA	20	20	50.0	50.0	58.0	58.4	15.2	0.0	0.440154	113817	116045	2842-2926	1.000000	77.8
ndhF_p5	ndhF	5	TTTAGGCAGAATACCGTCGC	CCGGAAGAAATAAGGGCTGG	20	20	50.0	55.0	58.1	58.3	0.0	0.0	0.389095	113817	116045	2988-2997	0.750000	68.2
ndhF-rpl32_p1	ndhF-rpl32	1	GGAACAAGCAGAAGTCCCAA	TACGAATCTTATCCCCGCCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024114	116046	117168	1842-1858	1.000000	83.0
ndhF-rpl32_p2	ndhF-rpl32	2	AGCAGAAGTCCCAACCCTAT	TACGAATCTTATCCCCGCCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032782	116046	117168	1836-1852	1.000000	83.0
ndhF-rpl32_p3	ndhF-rpl32	3	AATAGGGAGGCACACAGAGT	TACGAATCTTATCCCCGCCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.047082	116046	117168	2451-2467	1.000000	82.3
ndhF-rpl32_p4	ndhF-rpl32	4	AAGGGGAAATTGTGCGGATT	TACGAATCTTATCCCCGCCT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.068179	116046	117168	2491-2507	1.000000	82.1
ndhF-rpl32_p5	ndhF-rpl32	5	AGGGGAAATTGTGCGGATTT	TACGAATCTTATCCCCGCCT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.068179	116046	117168	2490-2506	1.000000	82.1
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	ACAAACAAGTAATAAAGCCTTGGA	AGCAGCGTGTCTACCAATTT	24	20	33.3	45.0	57.0	57.8	0.0	0.0	5.154997	117343	118745	1447-1463	1.000000	43.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	ACAAACAAGTAATAAAGCCTTGGA	GAGCAGCGTGTCTACCAATT	24	20	33.3	50.0	57.0	58.3	0.0	0.0	5.243140	117343	118745	1448-1464	1.000000	43.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	ACAAACAAGTAATAAAGCCTTGGA	TCCTAAGAGCAGCGTGTCTA	24	20	33.3	50.0	57.0	57.9	0.0	0.0	5.094270	117343	118745	1454-1470	1.000000	43.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	ACAAACAAGTAATAAAGCCTTGGA	CCTAAGAGCAGCGTGTCTAC	24	20	33.3	55.0	57.0	57.8	0.0	0.0	5.166288	117343	118745	1453-1469	1.000000	43.3
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	ACAAACAAGTAATAAAGCCTTGGA	CTAAGAGCAGCGTGTCTACC	24	20	33.3	55.0	57.0	57.8	0.0	0.0	5.166288	117343	118745	1452-1468	1.000000	43.3
ycf1_p1	ycf1	1	TGGATCAGTTGTAATTACATCGA	AGGCTTCCTACTGATTACCA	23	20	34.8	45.0	55.5	55.2	18.7	0.0	8.267420	132275	132394	237-237	1.000000	43.0
ycf1_p2	ycf1	2	TGGATCAGTTGTAATTACATCGA	AGGCTTCCTACTGATTACCAA	23	21	34.8	42.9	55.5	56.1	18.7	0.0	8.440682	132275	132394	237-237	1.000000	43.0
ycf1_p3	ycf1	3	TGGATCAGTTGTAATTACATCGAA	AGGCTTCCTACTGATTACCA	24	20	33.3	45.0	56.2	55.2	18.7	0.0	8.568399	132275	132394	237-237	1.000000	43.0
ycf1_p4	ycf1	4	TTGGATCAGTTGTAATTACATCGA	AGGCTTCCTACTGATTACCA	24	20	33.3	45.0	56.2	55.2	18.7	0.0	8.568399	132275	132394	238-238	1.000000	43.0
ycf1_p5	ycf1	5	TGGATCAGTTGTAATTACATCGA	AGGCTTCCTACTGATTACCAAA	23	22	34.8	40.9	55.5	56.8	18.7	0.0	8.690104	132275	132394	237-237	1.000000	43.0