Hotspot

ndhF

SSC · 115449-117698 · 2250 bp

Pi 0.0122

MarkerSeek score 52.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2250
variable_sites: 81
pi: 0.0122
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	AGAGCTGGTGAATCGGAAAC	50.0	50.0	58.0	57.9	2627-2747 bp	1.0	85.6
ndhF_p2	ACAGGAACAAGAGGGATCCA	AGAGCTGGTGAATCGGAAAC	50.0	50.0	58.0	57.9	2613-2733 bp	1.0	85.5
ndhF_p3	AAGAGGGATCCACCGAAGAA	AGAGCTGGTGAATCGGAAAC	50.0	50.0	58.0	57.9	2605-2725 bp	1.0	85.5
ndhF_p4	CCTTCCCCTTCCCTTTGTTC	AGAGCTGGTGAATCGGAAAC	55.0	50.0	58.1	57.9	2582-2702 bp	1.0	85.3
ndhF_p5	TTTAGGCAGAATACCGTCGC	AGAGCTGGTGAATCGGAAAC	50.0	50.0	58.1	57.9	2742-2862 bp	1.0	85.0