Hotspot

ccsA-ndhD

SSC · 120019-120255 · 237 bp

Back to Actaea

Pi 0.034
MarkerSeek score 67.00
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
237
variable_sites
23
pi
0.034
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGTTCATTCGCATTAACATCT TCCGGATTTCGTTCTCTCAC 36.4 50.0 56.4 57.4 329-393 bp 1.0 55.9
ccsA-ndhD_p2 TGGTTCATTCGCATTAACATCT CCGGATTTCGTTCTCTCACT 36.4 50.0 56.4 57.3 328-392 bp 1.0 55.9
ccsA-ndhD_p3 TGGTTCATTCGCATTAACATC TCCGGATTTCGTTCTCTCAC 38.1 50.0 54.9 57.4 329-393 bp 1.0 52.0
ccsA-ndhD_p4 TGGTTCATTCGCATTAACATC CCGGATTTCGTTCTCTCACT 38.1 50.0 54.9 57.3 328-392 bp 1.0 52.0
ccsA-ndhD_p5 ATGGTTCATTCGCATTAACATCT TCCGGATTTCGTTCTCTCAC 34.8 50.0 56.9 57.4 330-394 bp 1.0 51.8