Hotspot

ndhF-trnL-UAG

SSC · 117699-118841 · 1143 bp

Back to Actaea

Pi 0.0263
MarkerSeek score 72.40
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
1143
variable_sites
97
pi
0.0263
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 ACGAAAGGTATGATCCACGC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 971-1309 bp 1.0 89.9
ndhF-trnL-UAG_p2 CGAAAGGTATGATCCACGCA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 970-1308 bp 1.0 89.9
ndhF-trnL-UAG_p3 ACGAAAGGTATGATCCACGC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.1 58.0 972-1310 bp 1.0 89.9
ndhF-trnL-UAG_p4 CGAAAGGTATGATCCACGCA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.1 58.0 971-1309 bp 1.0 89.9
ndhF-trnL-UAG_p5 ACGAAAGGTATGATCCACGC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.1 57.9 981-1319 bp 1.0 88.9