Hotspot

rpl14-rpl16

LSC · 85641-85777 · 137 bp

Back to Actaea

Pi 0.0516
MarkerSeek score 70.80
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
137
variable_sites
22
pi
0.0516
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.96
markerseek_score
70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 CGCACCAAAAACTCGAGTTC CCTACCGAAACACGTATGGG 50.0 55.0 58.0 58.1 612-618 bp 1.0 84.5
rpl14-rpl16_p2 CGCACCAAAAACTCGAGTTC AATCGTTTCATTCGCGAGGA 50.0 45.0 58.0 58.0 927-933 bp 1.0 84.1
rpl14-rpl16_p3 CGCACCAAAAACTCGAGTTC GGTTCTGCAGTAGAGATGGC 50.0 55.0 58.0 58.1 880-886 bp 1.0 84.0
rpl14-rpl16_p4 CGCACCAAAAACTCGAGTTC TATGGGTTCGGGGAAAGGAT 50.0 50.0 58.0 58.1 598-604 bp 0.9 79.6
rpl14-rpl16_p5 CGCACCAAAAACTCGAGTTC ATGCACTTCAGGCACTTGAA 50.0 45.0 58.0 58.0 752-754 bp 0.2 48.1