Hotspot

petD-rpoA

LSC · 82275-82470 · 196 bp

Back to Actaea

Pi 0.0363
MarkerSeek score 70.70
Primer available yes
Label petD-rpoA

Feature Metadata

label_name
petD-rpoA
feature_type
igs
region
LSC
length_bp
196
variable_sites
18
pi
0.0363
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD-rpoA_p1 TTGATTGGTACCGCAGTAGC GGGCATTCTACAAAAACATTTCG 50.0 39.1 58.0 57.2 359-366 bp 1.0 56.4
petD-rpoA_p2 AGCCCTTTGGTTGGGTATTG GGGCATTCTACAAAAACATTTCG 50.0 39.1 58.1 57.2 342-349 bp 1.0 55.9
petD-rpoA_p3 GGTTGGGTATTGGAGCAACA GGGCATTCTACAAAAACATTTCG 50.0 39.1 58.1 57.2 334-341 bp 1.0 55.9
petD-rpoA_p4 TGGTTGGGTATTGGAGCAAC GGGCATTCTACAAAAACATTTCG 50.0 39.1 58.1 57.2 335-342 bp 1.0 55.9
petD-rpoA_p5 AGTAGCCCTTTGGTTGGGTA GGGCATTCTACAAAAACATTTCG 50.0 39.1 58.2 57.2 345-352 bp 1.0 54.4