Hotspot

psaJ-rpl33

LSC · 71731-72165 · 435 bp

Back to Actaea

Pi 0.0246
MarkerSeek score 68.20
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
435
variable_sites
27
pi
0.0246
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.97
markerseek_score
68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 ATCGGTTATTCCCAGATGCG AATGCCCCGCGATTTCTTAT 50.0 45.0 57.8 58.0 557-590 bp 1.0 85.2
psaJ-rpl33_p2 ATCGGTTATTCCCAGATGCG ATGCCCCGCGATTTCTTATT 50.0 45.0 57.8 58.0 556-589 bp 1.0 85.2
psaJ-rpl33_p3 TTCCCAGATGCGTTGACATT AATGCCCCGCGATTTCTTAT 45.0 45.0 57.8 58.0 549-582 bp 1.0 85.1
psaJ-rpl33_p4 TTCCCAGATGCGTTGACATT ATGCCCCGCGATTTCTTATT 45.0 45.0 57.8 58.0 548-581 bp 1.0 85.1
psaJ-rpl33_p5 TATCTCTCTGTGGCACCTGT ATGCCCCGCGATTTCTTATT 50.0 45.0 57.8 58.0 626-659 bp 1.0 83.9