Hotspot

petN-psbM

LSC · 30721-31961 · 1241 bp

Back to Actaea

Pi 0.0144
MarkerSeek score 68.50
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1241
variable_sites
57
pi
0.0144
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCTTGCTTGGGCTGCTTTAA TGCACTGTTCATTCTAGTCCC 45.0 47.6 58.0 57.7 1337-1390 bp 1.0 76.6
petN-psbM_p2 TCTTGCTTGGGCTGCTTTAA TGCTACTGCACTGTTCATTCT 45.0 42.9 58.0 57.6 1343-1396 bp 1.0 76.0
petN-psbM_p3 AGTAAGTCTTGCTTGGGCTG TGCACTGTTCATTCTAGTCCC 50.0 47.6 57.8 57.7 1343-1396 bp 1.0 75.1
petN-psbM_p4 AGTAAGTCTTGCTTGGGCTG TGCTACTGCACTGTTCATTCT 50.0 42.9 57.8 57.6 1349-1402 bp 1.0 74.5
petN-psbM_p5 TCTTGCTTGGGCTGCTTTAA GCACTGTTCATTCTAGTCCCT 45.0 47.6 58.0 57.4 1336-1389 bp 1.0 74.3