Hotspot

rps16-trnQ-UUG

LSC · 6307-8196 · 1890 bp

Back to Actaea

Pi 0.0242
MarkerSeek score 70.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1890
variable_sites
127
pi
0.0242
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.97
markerseek_score
70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ACCGATCATGTCCTTCAAGC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1921-2038 bp 1.0 88.3
rps16-trnQ-UUG_p2 CCACAACCGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1926-2043 bp 1.0 87.6
rps16-trnQ-UUG_p3 ACCGATCATGTCCTTCAAGC CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.0 57.8 1925-2042 bp 1.0 87.2
rps16-trnQ-UUG_p4 CCACAACCGATCATGTCCTT CTCGGAGGTTCGAATCCTTC 50.0 55.0 57.9 57.8 1930-2047 bp 1.0 86.5
rps16-trnQ-UUG_p5 ATCCACAACCGATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 1928-2045 bp 1.0 85.8