Hotspot

ycf1

SSC · 126215-131692 · 5478 bp

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Pi 0.00803
MarkerSeek score 43.70
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5478
variable_sites
44
pi
0.00803
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GTGTGACATTTCATTTTTAACAGCA TCGCTTTAAAGAGACATGCGA 32.0 42.9 57.5 58.0 241 bp 1.0 42.0
ycf1_p2 GTGTGACATTTCATTTTTAACAGCA AGCATGAATTCCACTTTCGC 32.0 45.0 57.5 57.1 257 bp 1.0 41.9
ycf1_p3 GTGTGACATTTCATTTTTAACAGCA GCATGAATTCCACTTTCGCT 32.0 45.0 57.5 57.1 256 bp 1.0 41.9
ycf1_p4 AGTGTGACATTTCATTTTTAACAGC AGCATGAATTCCACTTTCGC 32.0 45.0 57.3 57.1 258 bp 1.0 41.9
ycf1_p5 AGTGTGACATTTCATTTTTAACAGC GCATGAATTCCACTTTCGCT 32.0 45.0 57.3 57.1 257 bp 1.0 41.9