Hotspot

rpoC2

LSC · 16697-20902 · 4206 bp

Back to Acronychia

Pi 0.00571

MarkerSeek score 42.10

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4206
variable_sites: 24
pi: 0.00571
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	AGAATGGTGTTATGCTGCCT	AGTTTTCAAGAAACCACGCG	45.0	45.0	57.2	57.5	161 bp	1.0	72.2
rpoC2_p2	AAGAATGGTGTTATGCTGCCT	AGTTTTCAAGAAACCACGCG	42.9	45.0	57.9	57.5	162 bp	1.0	70.0
rpoC2_p3	AGAATGGTGTTATGCTGCCTT	AGTTTTCAAGAAACCACGCG	42.9	45.0	57.9	57.5	161 bp	1.0	70.0
rpoC2_p4	AGAATGGTGTTATGCTGCCT	AAGTTTTCAAGAAACCACGCG	45.0	42.9	57.2	58.2	162 bp	1.0	66.3
rpoC2_p5	GAATGGTGTTATGCTGCCTT	AGTTTTCAAGAAACCACGCG	45.0	45.0	56.4	57.5	160 bp	1.0	65.8