Hotspot

trnI-GAU-trnA-UGC

IRb · 105454-105517 · 64 bp

Back to Acronychia

Pi 0.0156

MarkerSeek score 61.50

Primer available yes

Label trnI-GAU-trnA-UGC

Feature Metadata

label_name: trnI-GAU-trnA-UGC
feature_type: igs
region: IRb
length_bp: 64
variable_sites: 1
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-GAU-trnA-UGC_p1	TGATTTACTTCACGGGCGAG	CAACGACCCAATTGCAAGAG	50.0	50.0	58.0	57.7	161 bp	1.0	77.8
trnI-GAU-trnA-UGC_p2	TGATTTACTTCACGGGCGAG	CCGCCTGGACAATTAGACAT	50.0	50.0	58.0	57.7	195 bp	1.0	77.7
trnI-GAU-trnA-UGC_p3	GCTCCCGAAAAGGAATCCAT	CCGCCTGGACAATTAGACAT	50.0	50.0	57.9	57.7	250 bp	1.0	77.2
trnI-GAU-trnA-UGC_p4	GCTCCCGAAAAGGAATCCAT	CAACGACCCAATTGCAAGAG	50.0	50.0	57.9	57.7	216 bp	1.0	77.2
trnI-GAU-trnA-UGC_p5	GCGAGGTCTCTGGTTCAAAT	CCGCCTGGACAATTAGACAT	50.0	50.0	57.9	57.7	180 bp	1.0	77.0