Hotspot

petL-petG

LSC · 67805-67994 · 190 bp

Back to Acronychia

Pi 0.0526

MarkerSeek score 71.00

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 190
variable_sites: 10
pi: 0.0526
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	AGCTCCGTCATGCCTACTAT	GGAATTAGACCTAAGACGATTCCA	50.0	41.7	58.0	57.8	339 bp	1.0	49.1
petL-petG_p2	TTCCTTTAGCTCCGTCATGC	GGAATTAGACCTAAGACGATTCCA	50.0	41.7	58.0	57.8	346 bp	1.0	48.9
petL-petG_p3	AGCTCCGTCATGCCTACTAT	AGACCTAAGACGATTCCAAA	50.0	40.0	58.0	53.7	333 bp	1.0	47.9
petL-petG_p4	TTCCTTTAGCTCCGTCATGC	AGACCTAAGACGATTCCAAA	50.0	40.0	58.0	53.7	340 bp	1.0	47.7
petL-petG_p5	TTAGCTCCGTCATGCCTACT	GGAATTAGACCTAAGACGATTCCA	50.0	41.7	58.2	57.8	341 bp	1.0	47.3