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psbE-petL

LSC · 66329-67708 · 1380 bp

Back to Acronychia

Pi 0.00291

MarkerSeek score 55.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1380
variable_sites: 4
pi: 0.00291
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AGTCCTATCTTGCTCAGACCA	45.0	47.6	58.0	57.9	1580-1584 bp	1.0	71.4
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGTCCTATCTTGCTCAGACCA	50.0	47.6	57.9	57.9	1569-1573 bp	1.0	71.1
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AGTCCTATCTTGCTCAGACCA	50.0	47.6	57.8	57.9	1492-1496 bp	1.0	70.3
psbE-petL_p4	TGACAAATAACCAACCCGCA	AAGCTGCTAGGAGAAAACCA	45.0	45.0	58.0	56.7	1534-1538 bp	1.0	70.3
psbE-petL_p5	TGACAAATAACCAACCCGCA	AGCTGCTAGGAGAAAACCAA	45.0	45.0	58.0	56.7	1533-1537 bp	1.0	70.3