Hotspot

psaI-ycf4

LSC · 61052-61519 · 468 bp

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Pi 0.00671

MarkerSeek score 62.80

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 468
variable_sites: 3
pi: 0.00671
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	AGTGGCAATGGCTTCGTTAT	AAAAAGGATAAGGGCCCAGC	45.0	50.0	57.9	58.1	586-607 bp	1.0	81.1
psaI-ycf4_p2	AGTCCCTTTAGTGGGCCTAG	AAAAAGGATAAGGGCCCAGC	55.0	50.0	58.1	58.1	616-637 bp	1.0	81.0
psaI-ycf4_p3	CCTAGTATTTCCGGCAGTGG	AAAAAGGATAAGGGCCCAGC	55.0	50.0	57.8	58.1	601-622 bp	1.0	80.1
psaI-ycf4_p4	TTAGTCCCTTTAGTGGGCCT	AAAAAGGATAAGGGCCCAGC	50.0	50.0	57.6	58.1	618-639 bp	1.0	79.1
psaI-ycf4_p5	CTAGTATTTCCGGCAGTGGC	AAAAAGGATAAGGGCCCAGC	55.0	50.0	58.4	58.1	600-621 bp	1.0	78.7