Hotspot

accD-psaI

LSC · 60203-60937 · 735 bp

Back to Acronychia

Pi 0.015

MarkerSeek score 67.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 11
pi: 0.015
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAAGGTGTTCTGAGCGAGT	ATAACGAAGCCATTGCCACT	45.0	45.0	57.4	57.9	884-887 bp	1.0	75.3
accD-psaI_p2	AAAGGTGTTCTGAGCGAGTT	ATAACGAAGCCATTGCCACT	45.0	45.0	57.4	57.9	883-886 bp	1.0	75.3
accD-psaI_p3	AAAAGGTGTTCTGAGCGAGT	CCACTGCCGGAAATACTAGG	45.0	55.0	57.4	57.8	869-872 bp	1.0	74.4
accD-psaI_p4	AAAGGTGTTCTGAGCGAGTT	CCACTGCCGGAAATACTAGG	45.0	55.0	57.4	57.8	868-871 bp	1.0	74.4
accD-psaI_p5	AAAGGTGTTCTGAGCGAGTT	GCCACTGCCGGAAATACTAG	45.0	55.0	57.4	58.4	869-872 bp	1.0	73.0