Hotspot

trnfM-CAU-rps14

LSC · 38392-38571 · 180 bp

Back to Acronychia

Pi 0.0303

MarkerSeek score 66.80

Primer available yes

Label trnfM-CAU-rps14

Feature Metadata

label_name: trnfM-CAU-rps14
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 5
pi: 0.0303
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-rps14_p1	AAGGTTATGAGCCTTGCGAG	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	328-343 bp	1.0	84.4
trnfM-CAU-rps14_p2	AAGGTTATGAGCCTTGCGAG	ACTATCCGGGCACAAACTTC	50.0	50.0	58.0	57.9	281-296 bp	1.0	84.3
trnfM-CAU-rps14_p3	AAGGTTATGAGCCTTGCGAG	GACTATCCGGGCACAAACTT	50.0	50.0	58.0	57.9	282-297 bp	1.0	84.3
trnfM-CAU-rps14_p4	TTTGAACCCGTGACCTCAAG	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	345-360 bp	1.0	84.1
trnfM-CAU-rps14_p5	TTTGAACCCGTGACCTCAAG	ACTATCCGGGCACAAACTTC	50.0	50.0	58.0	57.9	298-313 bp	1.0	84.1