Hotspot

petN-psbM

LSC · 29300-30576 · 1277 bp

Back to Acronychia

Pi 0.0105

MarkerSeek score 66.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1277
variable_sites: 13
pi: 0.0105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACATTTTCCCTTTCGCTCGT	TCGTTCTAGTTCCTACTGCCT	45.0	47.6	58.1	57.9	1356-1387 bp	1.0	73.2
petN-psbM_p2	CTTTCGCTCGTAGTATGGGG	TCGTTCTAGTTCCTACTGCCT	55.0	47.6	57.9	57.9	1347-1378 bp	1.0	73.0
petN-psbM_p3	CCCTTTCGCTCGTAGTATGG	TCGTTCTAGTTCCTACTGCCT	55.0	47.6	57.9	57.9	1349-1380 bp	1.0	72.9
petN-psbM_p4	CCTTTCGCTCGTAGTATGGG	TCGTTCTAGTTCCTACTGCCT	55.0	47.6	57.9	57.9	1348-1379 bp	1.0	72.9
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	TCGTTCTAGTTCCTACTGCCT	50.0	47.6	58.2	57.9	1394-1425 bp	1.0	72.7