Hotspot

ycf1

SSC · 124944-130406 · 5463 bp

Back to Acronema

Pi 0.0271

MarkerSeek score 68.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5463
variable_sites: 640
pi: 0.0271
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTTTTCTTGGCGTTACCGT	GAAACCTTGGCACAGATCCA	45.0	50.0	57.8	58.1	571-580 bp	0.529412	67.5
ycf1_p2	TCTTGGCGTTACCGTTTTCT	GAAACCTTGGCACAGATCCA	45.0	50.0	57.8	58.1	575 bp	0.176471	45.6
ycf1_p3	TCTTGGCGTTACCGTTTTCT	TGAAACCTTGGCACAGATCC	45.0	50.0	57.8	58.1	576 bp	0.176471	45.6
ycf1_p4	TGGCGTTACCGTTTTCTTCT	GAAACCTTGGCACAGATCCA	45.0	50.0	57.8	58.1	572 bp	0.176471	45.6
ycf1_p5	TGGCGTTACCGTTTTCTTCT	TGAAACCTTGGCACAGATCC	45.0	50.0	57.8	58.1	573 bp	0.176471	45.6