Hotspot

rpl32-trnL-UAG

SSC · 114380-115311 · 932 bp

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Pi 0.0592
MarkerSeek score 80.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
932
variable_sites
221
pi
0.0592
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.95
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ATTGGGCAGCCTTAAAAGCT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 967-1094 bp 0.823529 84.4
rpl32-trnL-UAG_p2 ATTGGGCAGCCTTAAAAGCT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.1 58.0 968-1095 bp 0.823529 84.4
rpl32-trnL-UAG_p3 GGGCAGCCTTAAAAGCTTTG AGCGTGTCTACCGATTTCAC 50.0 50.0 57.9 58.0 964-1091 bp 0.823529 84.2
rpl32-trnL-UAG_p4 GGGCAGCCTTAAAAGCTTTG CAGCGTGTCTACCGATTTCA 50.0 50.0 57.9 58.0 965-1092 bp 0.823529 84.2
rpl32-trnL-UAG_p5 ATTGGGCAGCCTTAAAAGCT CTCGCACTGCTTCCTAAGAG 45.0 55.0 58.1 58.1 988-1115 bp 0.823529 83.8