Hotspot

rbcL-accD

LSC · 57647-58253 · 607 bp

Back to Acronema

Pi 0.0291
MarkerSeek score 76.70
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
607
variable_sites
76
pi
0.0291
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 TGAATTTCAAGCAATGGATACT ATCGAACCACCATCTTTCCA 31.8 45.0 54.0 56.8 646-693 bp 0.941176 48.9
rbcL-accD_p2 TGAATTTCAAGCAATGGATACT TCGAACCACCATCTTTCCAT 31.8 45.0 54.0 56.8 645-692 bp 0.941176 48.9
rbcL-accD_p3 TGAATTTCAAGCAATGGATACTT ATCGAACCACCATCTTTCCA 30.4 45.0 54.8 56.8 646-693 bp 0.941176 48.9
rbcL-accD_p4 TGAATTTCAAGCAATGGATACTT TCGAACCACCATCTTTCCAT 30.4 45.0 54.8 56.8 645-692 bp 0.941176 48.9
rbcL-accD_p5 TTGAATTTCAAGCAATGGATACT TCGAACCACCATCTTTCCAT 30.4 45.0 54.8 56.8 646-693 bp 0.941176 48.9