Hotspot

psbM-trnD-GUC

LSC · 30564-31184 · 621 bp

Back to Acronema

Pi 0.0372
MarkerSeek score 75.70
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
621
variable_sites
107
pi
0.0372
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AAATGCAAGAATATTTACTTCCA GTTCGAGCCCCGTCAGTC 26.1 66.7 52.7 60.1 620-752 bp 1.0 52.0
psbM-trnD-GUC_p2 AATGCAAGAATATTTACTTCCAT GTTCGAGCCCCGTCAGTC 26.1 66.7 52.4 60.1 619-751 bp 1.0 52.0
psbM-trnD-GUC_p3 AAATGCAAGAATATTTACTTCCAT GTTCGAGCCCCGTCAGTC 25.0 66.7 53.3 60.1 620-752 bp 1.0 52.0
psbM-trnD-GUC_p4 AAATGCAAGAATATTTACTTCCA GAGCACCGCCCTGTCAAG 26.1 66.7 52.7 60.7 651-783 bp 1.0 52.0
psbM-trnD-GUC_p5 AAATGCAAGAATATTTACTTCCA GCCCTGTCAAGGCGGAAG 26.1 66.7 52.7 60.7 644-776 bp 1.0 52.0