Hotspot

trnC-GCA-petN

LSC · 28604-29239 · 636 bp

Back to Acronema

Pi 0.0377

MarkerSeek score 81.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 636
variable_sites: 99
pi: 0.0377
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	CGATATGGCCGAGTGGTAAG	AGCCCAAGCGAGACTTACTA	55.0	50.0	57.9	58.2	455-813 bp	1.0	90.3
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	415-773 bp	1.0	90.1
trnC-GCA-petN_p3	CGATATGGCCGAGTGGTAAG	AGAGTCCACTTCTTCCCCAT	55.0	50.0	57.9	57.6	516-874 bp	1.0	88.7
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	476-834 bp	1.0	88.5
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	433-791 bp	1.0	87.9