Hotspot

rps16-trnQ-UUG

LSC · 6003-7411 · 1409 bp

Back to Acronema

Pi 0.0436
MarkerSeek score 76.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1409
variable_sites
233
pi
0.0436
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CGCGCGATACACTTTGAATC TACAGCAGCTTGCCAAATGA 50.0 45.0 58.0 58.1 2385 bp 0.117647 43.9
rps16-trnQ-UUG_p2 GCGCGATACACTTTGAATCG TACAGCAGCTTGCCAAATGA 50.0 45.0 58.0 58.1 2384 bp 0.117647 43.9
rps16-trnQ-UUG_p3 TGCTTTCTACCACAGCGTTT TACAGCAGCTTGCCAAATGA 45.0 45.0 58.0 58.1 2044 bp 0.117647 43.7
rps16-trnQ-UUG_p4 TTGCTTTCTACCACAGCGTT TACAGCAGCTTGCCAAATGA 45.0 45.0 58.0 58.1 2045 bp 0.117647 43.7
rps16-trnQ-UUG_p5 AAGTTTTCTCCTCGTACGGC TACAGCAGCTTGCCAAATGA 50.0 45.0 57.9 58.1 2745 bp 0.117647 43.4