Hotspot

trnK-UUU-rps16

LSC · 4273-5024 · 752 bp

Back to Acronema

Pi 0.0492

MarkerSeek score 71.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 752
variable_sites: 131
pi: 0.0492
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	GGCGGAGGTTTTTAAGTAG	50.0	47.4	58.2	53.4	566-951 bp	1.0	52.0
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	GGCGGAGGTTTTTAAGTAG	50.0	47.4	58.2	53.4	565-950 bp	1.0	52.0
trnK-UUU-rps16_p3	TCCTAGCCGCACTTAAAAGC	GGCGGAGGTTTTTAAGTAG	50.0	47.4	58.3	53.4	581-966 bp	1.0	52.0
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	GGCGGAGGTTTTTAAGTAG	50.0	47.4	58.3	53.4	575-960 bp	1.0	52.0
trnK-UUU-rps16_p5	TACCGTTGAGTTAGCAACCC	GGCGGAGGTTTTTAAGTAG	50.0	47.4	57.5	53.4	552-937 bp	1.0	52.0