primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	CAATCCACTGCCTTGATCCA	AGCTCCATCTACAAATGGGT	20	20	50.0	45.0	57.9	56.2	0.0	3.2	1.941039	287	459	220-449	1.000000	76.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	AATCCACTGCCTTGATCCAC	AGCTCCATCTACAAATGGGT	20	20	50.0	45.0	57.9	56.2	0.0	3.2	1.942547	287	459	219-448	1.000000	76.5
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	ACAATCCACTGCCTTGATCC	AGCTCCATCTACAAATGGGT	20	20	50.0	45.0	57.9	56.2	0.0	3.2	1.942547	287	459	221-450	1.000000	76.5
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	GATCCACTTGGCTACATCCG	AGCTCCATCTACAAATGGGT	20	20	55.0	45.0	58.1	56.2	0.0	3.2	1.936678	287	459	206-435	0.941176	74.2
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	AAGCTCCATCTACAAATGGGT	20	21	55.0	42.9	58.1	57.0	0.0	3.2	2.158020	287	459	207-436	0.941176	72.4
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	GGCGGAGGTTTTTAAGTAG	20	19	50.0	47.4	58.2	53.4	20.3	0.0	5.776233	4273	5024	566-951	1.000000	52.0
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	GGCGGAGGTTTTTAAGTAG	20	19	50.0	47.4	58.2	53.4	20.3	0.0	5.776233	4273	5024	565-950	1.000000	52.0
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCCTAGCCGCACTTAAAAGC	GGCGGAGGTTTTTAAGTAG	20	19	50.0	47.4	58.3	53.4	0.0	0.0	5.873651	4273	5024	581-966	1.000000	52.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCGCACTTAAAAGCCGAGTA	GGCGGAGGTTTTTAAGTAG	20	19	50.0	47.4	58.3	53.4	5.3	0.0	5.890619	4273	5024	575-960	1.000000	52.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TACCGTTGAGTTAGCAACCC	GGCGGAGGTTTTTAAGTAG	20	19	50.0	47.4	57.5	53.4	0.0	0.0	6.068570	4273	5024	552-937	1.000000	52.0
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CGCGCGATACACTTTGAATC	TACAGCAGCTTGCCAAATGA	20	20	50.0	45.0	58.0	58.1	28.9	0.0	0.099831	6003	7411	2385-2385	0.117647	43.9
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GCGCGATACACTTTGAATCG	TACAGCAGCTTGCCAAATGA	20	20	50.0	45.0	58.0	58.1	12.1	0.0	0.099831	6003	7411	2384-2384	0.117647	43.9
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TGCTTTCTACCACAGCGTTT	TACAGCAGCTTGCCAAATGA	20	20	45.0	45.0	58.0	58.1	0.0	0.0	0.129863	6003	7411	2044-2044	0.117647	43.7
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TTGCTTTCTACCACAGCGTT	TACAGCAGCTTGCCAAATGA	20	20	45.0	45.0	58.0	58.1	0.0	0.0	0.129863	6003	7411	2045-2045	0.117647	43.7
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTTTTCTCCTCGTACGGC	TACAGCAGCTTGCCAAATGA	20	20	50.0	45.0	57.9	58.1	16.5	0.0	0.167635	6003	7411	2745-2745	0.117647	43.4
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TTCTAATTGGTCAGGAGCGC	20	20	45.0	50.0	58.0	58.0	1.4	0.0	0.062562	13842	14978	1279-1631	1.000000	91.5
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	CTAATTGGTCAGGAGCGCTT	20	20	45.0	50.0	58.0	58.0	1.4	13.3	0.068225	13842	14978	1277-1629	1.000000	91.5
atpH-atpI_p3	atpH-atpI	3	ATAACGGAAGCGGCAGAAAT	TGCTTTGGCTTTACTCACGT	20	20	45.0	45.0	58.0	58.0	1.4	0.0	0.076593	13842	14978	1186-1538	1.000000	91.4
atpH-atpI_p4	atpH-atpI	4	CTCGCGATACCTTCTACAGC	TTCTAATTGGTCAGGAGCGC	20	20	55.0	50.0	58.0	58.0	10.4	0.0	0.063502	13842	14978	1369-1721	0.941176	89.1
atpH-atpI_p5	atpH-atpI	5	CTCGCGATACCTTCTACAGC	CTAATTGGTCAGGAGCGCTT	20	20	55.0	50.0	58.0	58.0	10.4	13.3	0.069165	13842	14978	1367-1719	0.941176	89.1
rps2-rpoC2_p1	rps2-rpoC2	1	AATGAACTCCGGCCTTCATC	TTGGGGGTTCTTGATTGGTG	20	20	50.0	50.0	58.0	58.0	20.5	0.0	0.052365	16696	16926	889-922	1.000000	89.8
rps2-rpoC2_p2	rps2-rpoC2	2	AACATAATGACACCGAGCCC	TTGGGGGTTCTTGATTGGTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.046629	16696	16926	1119-1152	1.000000	89.2
rps2-rpoC2_p3	rps2-rpoC2	3	CCGCATCTCTTTTCGGAAGA	TTGGGGGTTCTTGATTGGTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.018388	16696	16926	1246-1279	0.882353	81.6
rps2-rpoC2_p4	rps2-rpoC2	4	CCGCATCTCTTTTCGGAAGA	GAGGGTTGGCACGAATGTAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048532	16696	16926	1287-1320	0.882353	81.3
rps2-rpoC2_p5	rps2-rpoC2	5	CCGCATCTCTTTTCGGAAGA	GATTCGAGGGATTGGTGCAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.037273	16696	16926	828-834	0.176471	47.7
trnC-GCA-petN_p1	trnC-GCA-petN	1	CGATATGGCCGAGTGGTAAG	AGCCCAAGCGAGACTTACTA	20	20	55.0	50.0	57.9	58.2	0.0	0.0	0.217341	28604	29239	455-813	1.000000	90.3
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	28604	29239	415-773	1.000000	90.1
trnC-GCA-petN_p3	trnC-GCA-petN	3	CGATATGGCCGAGTGGTAAG	AGAGTCCACTTCTTCCCCAT	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.416855	28604	29239	516-874	1.000000	88.7
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.432990	28604	29239	476-834	1.000000	88.5
trnC-GCA-petN_p5	trnC-GCA-petN	5	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	28604	29239	433-791	1.000000	87.9
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AAATGCAAGAATATTTACTTCCA	GTTCGAGCCCCGTCAGTC	23	18	26.1	66.7	52.7	60.1	0.0	0.0	12.465267	30564	31184	620-752	1.000000	52.0
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AATGCAAGAATATTTACTTCCAT	GTTCGAGCCCCGTCAGTC	23	18	26.1	66.7	52.4	60.1	0.0	0.0	12.683110	30564	31184	619-751	1.000000	52.0
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AAATGCAAGAATATTTACTTCCAT	GTTCGAGCCCCGTCAGTC	24	18	25.0	66.7	53.3	60.1	0.0	0.0	12.846628	30564	31184	620-752	1.000000	52.0
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AAATGCAAGAATATTTACTTCCA	GAGCACCGCCCTGTCAAG	23	18	26.1	66.7	52.7	60.7	0.0	0.0	13.083164	30564	31184	651-783	1.000000	52.0
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AAATGCAAGAATATTTACTTCCA	GCCCTGTCAAGGCGGAAG	23	18	26.1	66.7	52.7	60.7	0.0	0.0	13.087091	30564	31184	644-776	1.000000	52.0
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	GTGCTCTGACCGATTGAACT	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.092025	31613	32337	1050-1450	1.000000	91.3
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	31613	32337	717-1123	1.000000	90.6
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	GTGCTCTGACCGATTGAACT	CACGAAGCTGCGATCTGATA	20	20	50.0	50.0	57.9	57.9	0.0	24.0	0.211516	31613	32337	1674-2136	1.000000	90.3
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	GTGCTCTGACCGATTGAACT	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.236958	31613	32337	1047-1447	1.000000	90.1
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GTGCTCTGACCGATTGAACT	TGGTGATTCCAGAGACGACT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.161323	31613	32337	1246-1620	0.941176	88.4
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	45325	46201	375-1005	1.000000	82.5
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	21	20	47.6	45.0	58.0	57.8	8.2	0.0	1.233148	45325	46201	325-955	1.000000	82.1
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	21	20	47.6	50.0	58.0	58.3	8.2	0.0	1.301418	45325	46201	327-957	1.000000	81.6
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	58.0	58.1	8.2	0.0	1.134847	45325	46201	354-984	0.941176	80.6
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	21	20	47.6	50.0	58.0	58.4	8.2	0.0	1.427808	45325	46201	355-985	0.941176	78.2
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	TGGATAGGATGGCCTTTACG	20	20	55.0	50.0	58.0	56.8	10.3	3.3	1.224095	49408	49793	518-543	1.000000	82.0
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	GGGATAGCTCAGCTGGTAGA	GGATAGGATGGCCTTTACGT	20	20	55.0	50.0	58.0	56.8	10.3	3.3	1.226313	49408	49793	517-542	1.000000	82.0
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.273485	49408	49793	482-507	1.000000	81.8
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.275704	49408	49793	481-506	1.000000	81.8
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CTGGTAGAGCAGAGGACTGA	TGGATAGGATGGCCTTTACG	20	20	55.0	50.0	57.9	56.8	2.5	3.3	1.357888	49408	49793	506-531	1.000000	81.0
rbcL-accD_p1	rbcL-accD	1	TGAATTTCAAGCAATGGATACT	ATCGAACCACCATCTTTCCA	22	20	31.8	45.0	54.0	56.8	0.0	0.0	7.127817	57647	58253	646-693	0.941176	48.9
rbcL-accD_p2	rbcL-accD	2	TGAATTTCAAGCAATGGATACT	TCGAACCACCATCTTTCCAT	22	20	31.8	45.0	54.0	56.8	0.0	0.0	7.127817	57647	58253	645-692	0.941176	48.9
rbcL-accD_p3	rbcL-accD	3	TGAATTTCAAGCAATGGATACTT	ATCGAACCACCATCTTTCCA	23	20	30.4	45.0	54.8	56.8	0.0	0.0	7.329778	57647	58253	646-693	0.941176	48.9
rbcL-accD_p4	rbcL-accD	4	TGAATTTCAAGCAATGGATACTT	TCGAACCACCATCTTTCCAT	23	20	30.4	45.0	54.8	56.8	0.0	0.0	7.329778	57647	58253	645-692	0.941176	48.9
rbcL-accD_p5	rbcL-accD	5	TTGAATTTCAAGCAATGGATACT	TCGAACCACCATCTTTCCAT	23	20	30.4	45.0	54.8	56.8	7.7	0.0	7.329778	57647	58253	646-693	0.941176	48.9
petA-psbJ_p1	petA-psbJ	1	CAGGGCCAGAACTTCTTGTT	AACTGGCCGTTTTGATCCTT	20	20	50.0	45.0	58.0	58.0	6.2	0.0	0.017741	63722	64692	1605-1804	1.000000	91.9
petA-psbJ_p2	petA-psbJ	2	CAGGGCCAGAACTTCTTGTT	ACTGGCCGTTTTGATCCTTT	20	20	50.0	45.0	58.0	58.0	6.2	0.0	0.017741	63722	64692	1604-1803	1.000000	91.9
petA-psbJ_p3	petA-psbJ	3	CGGATTTGGTCAGGGAGATG	AACTGGCCGTTTTGATCCTT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.045582	63722	64692	1529-1728	0.882353	86.9
petA-psbJ_p4	petA-psbJ	4	CGGATTTGGTCAGGGAGATG	ACTGGCCGTTTTGATCCTTT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.045582	63722	64692	1528-1727	0.882353	86.9
petA-psbJ_p5	petA-psbJ	5	CAGGGCCAGAACTTCTTGTT	TGCGGGTTGGTTATTTGTCA	20	20	50.0	45.0	58.0	58.0	6.2	0.0	0.030457	63722	64692	1884-1884	0.117647	44.5
ycf2-trnL-CAA_p1	ycf2-trnL-CAA	1	CAGCCCTTTGTCTCTGTGTT	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.0	58.0	0.0	15.9	0.034958	93192	93857	591-1167	0.588235	70.4
ycf2-trnL-CAA_p2	ycf2-trnL-CAA	2	CAGCCCTTTGTCTCTGTGTT	TGAAATGGTAGACACGCGAG	20	20	50.0	50.0	58.0	58.0	0.0	15.9	0.036329	93192	93857	593-1169	0.588235	70.4
ycf2-trnL-CAA_p3	ycf2-trnL-CAA	3	GTTCTTGCAGAGTGGAACCA	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.0	58.0	0.0	15.9	0.040512	93192	93857	715-1291	0.588235	70.2
ycf2-trnL-CAA_p4	ycf2-trnL-CAA	4	GGCAAGCGGATCCTTTATGA	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.0	58.0	10.8	15.9	0.040512	93192	93857	768-1344	0.588235	70.0
ycf2-trnL-CAA_p5	ycf2-trnL-CAA	5	TAGCCGGAAAGTTATGTGGC	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.0	58.0	5.0	15.9	0.040511	93192	93857	760-797	0.529412	67.4
ndhF-rpl32_p1	ndhF-rpl32	1	AAGCGGGTCGATCAAGTAAC	ATTCCAGGCTTTTGTCGGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.010594	113090	114214	1286-2156	0.764706	82.5
ndhF-rpl32_p2	ndhF-rpl32	2	CCGTGTGAACCAAAACCCTA	ATTCCAGGCTTTTGTCGGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.034532	113090	114214	1514-2384	0.764706	82.3
ndhF-rpl32_p3	ndhF-rpl32	3	AAGCGGGTCGATCAAGTAAC	AATTCCAGGCTTTTGTCGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.010594	113090	114214	1287-2157	0.705882	80.2
ndhF-rpl32_p4	ndhF-rpl32	4	CCGTGTGAACCAAAACCCTA	AATTCCAGGCTTTTGTCGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.034532	113090	114214	1515-2385	0.705882	79.9
ndhF-rpl32_p5	ndhF-rpl32	5	GTTGCCGACGGAAAAAGAAG	ATTCCAGGCTTTTGTCGGTT	20	20	50.0	45.0	58.0	58.0	20.5	0.0	0.037038	113090	114214	1108-1978	0.705882	79.9
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	ATTGGGCAGCCTTAAAAGCT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.1	58.0	12.4	0.0	0.065054	114380	115311	967-1094	0.823529	84.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	ATTGGGCAGCCTTAAAAGCT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.1	58.0	12.4	0.0	0.066425	114380	115311	968-1095	0.823529	84.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGGCAGCCTTAAAAGCTTTG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.9	58.0	13.5	0.0	0.092440	114380	115311	964-1091	0.823529	84.2
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGGCAGCCTTAAAAGCTTTG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.9	58.0	13.5	0.0	0.093810	114380	115311	965-1092	0.823529	84.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	ATTGGGCAGCCTTAAAAGCT	CTCGCACTGCTTCCTAAGAG	20	20	45.0	55.0	58.1	58.1	12.4	0.0	0.137306	114380	115311	988-1115	0.823529	83.8
ycf1_p1	ycf1	1	TCTTTTCTTGGCGTTACCGT	GAAACCTTGGCACAGATCCA	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.333902	127620	127739	571-580	0.529412	67.5
ycf1_p2	ycf1	2	TCTTGGCGTTACCGTTTTCT	GAAACCTTGGCACAGATCCA	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.333902	127620	127739	575-575	0.176471	45.6
ycf1_p3	ycf1	3	TCTTGGCGTTACCGTTTTCT	TGAAACCTTGGCACAGATCC	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.333902	127620	127739	576-576	0.176471	45.6
ycf1_p4	ycf1	4	TGGCGTTACCGTTTTCTTCT	GAAACCTTGGCACAGATCCA	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.333902	127620	127739	572-572	0.176471	45.6
ycf1_p5	ycf1	5	TGGCGTTACCGTTTTCTTCT	TGAAACCTTGGCACAGATCC	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.333902	127620	127739	573-573	0.176471	45.6
trnL-CAA-ycf2_p1	trnL-CAA-ycf2	1	AAATGGTAGACACGCGAGAC	CAGCCCTTTGTCTCTGTGTT	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.034958	145528	146193	591-1167	0.588235	70.4
trnL-CAA-ycf2_p2	trnL-CAA-ycf2	2	TGAAATGGTAGACACGCGAG	CAGCCCTTTGTCTCTGTGTT	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.036329	145528	146193	593-1169	0.588235	70.4
trnL-CAA-ycf2_p3	trnL-CAA-ycf2	3	AAATGGTAGACACGCGAGAC	GTTCTTGCAGAGTGGAACCA	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.040512	145528	146193	715-1291	0.588235	70.2
trnL-CAA-ycf2_p4	trnL-CAA-ycf2	4	AAATGGTAGACACGCGAGAC	GGCAAGCGGATCCTTTATGA	20	20	50.0	50.0	58.0	58.0	15.9	10.8	0.040512	145528	146193	768-1344	0.588235	70.0
trnL-CAA-ycf2_p5	trnL-CAA-ycf2	5	AAATGGTAGACACGCGAGAC	TAGCCGGAAAGTTATGTGGC	20	20	50.0	50.0	58.0	58.0	15.9	5.0	0.040511	145528	146193	760-797	0.529412	66.8